Double Beam Uv-Visible Spectrophotometer - PerkinElmer LAMBDA 365 User Manual

10 . Lambda 365 Users Guide
Figure 4. Transmittance and Absorbance
Double Beam UV-Visible Spectrophotom eter
Lambda 365 double-beam UV-Visible spectrophotometer, the light is split into two beams
before it reaches the sample. One beam is used as the reference; the other beam passes
through the sample. The reference beam intensity is taken as 100% Transmission (or 0
Absorbance), and the measurement displayed is the ratio of the two beam intensities.
Lambda 365 double-beam UV-Visible spectrophotometer has two detectors (photodiodes),
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and the sample and reference beam are measured at the same time