Download
Share
Print this page
PerkinElmer Lambda 465 Installation Instructions Manual
  1. Manuals
  2. Brands
  3. PerkinElmer Manuals
  4. Laboratory Equipment
  5. Lambda 465
  6. Installation instructions manual
PerkinElmer Lambda 465 Installation Instructions Manual

PerkinElmer Lambda 465 Installation Instructions Manual

Peltier control single cell with peltier controller
Hide thumbs Also See for Lambda 465:

Advertisement

Quick Links

Download this manual
Lambda 465 Peltier Control Single Cell with Peltier
This instruction sheet describes the installation of this accessory which is used with the Lambda 465
Spectrophotometer.
NOTE: Read these instructions before you install this accessory.
Contacting P erk inElm er
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
•
Easy to install
•
Full software control
•
Liquid cooling system
•
N
gas purging available
2
Figure 1. Lambda 465 Peltier control Single Cell [P/N: N4104009]
Controller Installation Instructions
09931238D

Advertisement

loading

Related Manuals for PerkinElmer Lambda 465

Summary of Contents for PerkinElmer Lambda 465

  • Page 1 09931238D Lambda 465 Peltier Control Single Cell with Peltier Controller Installation Instructions This instruction sheet describes the installation of this accessory which is used with the Lambda 465 Spectrophotometer. NOTE: Read these instructions before you install this accessory. Contacting P erk inElm er Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part numbers.

  • Page 2: Dimensions And Specifications

    09931238D Dim ensions and Specifications Dimensions of Peltier Controller Physical Characteristic Specification Power 100-240 VAC, 50/60 Hz, 300W, Free Voltage Temperature Range -10 to 100°C (Maximum Internal Temperature) Maximum Ambient Operating Temperature 40°C Dimensions 310 (W) x 410 (D) x 275 (H) Weight 20.0 kg Coolant Volume...

  • Page 3: Safety Warnings

    09931238D Safety W arnings When this label is attached to an instrument it means refer to the manual. Lorsque cette étiquette est attachée à un instrument, il est nécessaire WARNING de voir le manuel. See the following figure for the location of warning labels on the back of the instrument: Warning To prevent electrical shock, do not open cover.
  • Page 4 09931238D Description Configuration of the Peltier Control Single Cell Holder Figure 2 Lambda 465 Peltier Control Single Cell a) Interface connector between Peltier Single Cell Holder and Peltier Controller b) Coolant Inlet/Outlet Quick Coupler c) N gas fitting d) Interface slot for Temperature Probe...
  • Page 5 09931238D Figure 5 Wrench bolt M5, Length 15 mm, 2 ea Figure 6 Phillips round head screw with washer M4, 15 mm, 4 ea Front View of Peltier Controller Figure 7 Front view of the Peltier Controller a. Manual touch pad with Display: Temperature Control Unit (Heating/Cooling Block) b.
  • Page 6 09931238D R ear View of Peltier Controller Figure 8 Rear view of the Peltier Controller a. RS-232 port b. AC POWER: Main Power ON/OFF c. FUSE: AC Socket + Fuse Holder d. INTERFACE: Interface cable for connecting with Peltier control Single Cell e.

  • Page 7: Configuration Of The Equipm Ent

    Assurez-vous que l’instrument est éteint lors de l’installation de cet accessoire. 1. Place the Lambda 465, Peltier Controller and Peltier Control Single Cell in a location that is compatible with the required environmental conditions for the operation. 2. Open the lid of the coolant inlet on top of the Peltier Controller.
  • Page 8 à base d'eau distillée à 70 % M/V, de propylène glycol à 27 % M/V et d'additifs à 3 % M/V). 4. Connect the power cord and the communication cable of the Lambda 465. Make sure that the instrument is turned off when the accessory is installed.
  • Page 9 09931238D 6. Place the base plate at the bottom of the sample compartment and tighten the bolt with a wrench. Next fix and tighten the Peltier Control Single Cell with the Phillips round screw. Figure 11 Placing the plate at the bottom of the sample compartment 7.
  • Page 10 09931238D 8. Connect the coolant inlet/outlet and N gas tube between the Peltier Controller and the Peltier Control Single Cell. NOTE: gas is not always required, so the gas and inlet hose are not supplied. Figure 13 Connecting the coolant inlet/outlet and N gas tube 9.
  • Page 11 11. Connect the power cord to the Peltier Controller. Figure 16 Connecting the power cord 12. Turn on the power of the Lambda 465 and the Peltier Controller. Figure 17 Back of the instrument connections The Air vent manual button should be off before turning on the main power of the...
  • Page 12 09931238D Peltier controller auto tuning setup Peltier controller can be compatible with various peltier cell holders (Single or Multi) so whenever you exchange the existing peltier cell holder to the other one, you should proceed “AUTO TUNING” set up to minimize the temperature fluctuation at the target temperature 1.
  • Page 13 09931238D 4. Push the SET/ENT button again. 5. Push the button (OFF ON). 6. Press the SET/ENT button, MAN LED will blink. 7. MAN LED will be off after a few minutes.
  • Page 14 09931238D 8. Push Manual Button (Stop) and then light goes off. If you hear the alarm sound during the operation, push the emergency switch to CAUTION stop all functions immediately and check the status of Peltier Controller carefully. Then, turn the switch to the right again when everything is solved properly and the functions will be on again.
  • Page 15 When using the RS-232 cable, install the COM port drive properly according to the following procedure. Turn on the computer and the Peltier controller. Connect the USB to RS-232 cable between the computer and the Peltier controller. Select C>UV Lab> USB Drivers> Lambda 465> USB2 folder. Double click USBCom-CDM_20828. Select Extract.
  • Page 16 09931238D The following dialog box will appear. After the installation is completed successfully, select Finish. Change the USB serial ports after finishing driver setup. See the following section. Setting USB to R S-232 port Change the port setting as follow when communication is not connected by using the USB to RS-232 convert.
  • Page 17 09931238D Select Device Manager. Select Ports (COM & LPT) to expand the listing. These are the devices currently connected to the COM ports. USB Serial Port (COMx) is visible when the driver installation is completed successfully.
  • Page 18 09931238D Double click on the USB Serial Port (COMx) of the Ports (COM & LPT) section. Select the Port Settings tab and select the Advanced… button.
  • Page 19 09931238D Change the parameter values as shown in the following screen. Select OK after checking the changed parameter values. If the Peltier controller fails to communicate with the PC, change the COM Port Number in the following steps. Open the Advanced Setting for COMx window following steps 1 to 6 in this procedure.
  • Page 20 09931238D Select the COM Port number list to expand it and change the COM port number another one which is not in use from COM 1 to COM 10. Make sure that the changed COM Port Number is applied, and select OK. After the port setting is changed, restart the computer.
  • Page 21 09931238D M easurem ent : Start the sample measurement after warming up the system for at least 20 minutes. NOTE This accessory can be operated in Temperature Based Kinetics in the Kinetics mode and Thermal Denaturation in the Bio analysis mode. Tem perature Based K inetics Launch UV Lab software.
  • Page 22 09931238D - Start Temperature: Enter the start temperature for the measurement. - End Temperature: Enter the end temperature for the measurement. - Rate (°C/min): Enter the heating (cooling) rate. b. Holding Time: Enter the holding time before sample measurement after it reaches the each set temperature.

  • Page 23: Temperature Control

    09931238D The Setup Single Cell Peltier window will be shown. Set parameters according to the experiment conditions.  Temperature Control a. Temperature (°C): Enter the preset temperature for the experiment. NOTE: Starting the temperature of experiment needs to be set up in the method window. b.
  • Page 24 09931238D  Option: This function is used to adjust temperature. It is only used for the manufacturing process, so do not modify the values. After completing the parameter setup, select OK. Then the LED for On-LINE is turned on and it will start heating up or cooling down to the set temperature in the Setup Single Cell Peltier.
  • Page 25 09931238D a. Start Temp (°C): Shows starting temperature of the experiment. b. Measurement Temp. (°C): Shows the temperature at which the sample will be measured. c. SP Temp (°C): Shows the current temperature while it goes up/down to the measurement temperature.
  • Page 26 09931238D The following dialog box will be displayed. Setup test parameters as follows: a. Edit Heating (Cooling) Rate (°C/min): Set the heating (cooling) temperature rate in each temperature range. Sample is measured whenever it reaches the set temperature by the Rate (°C/min), and the sample is also measured at the Start Temperature and the End Temperature.
  • Page 27 09931238D Volume (T) = 0.99829+104.5 * 10 T+3.5 * 10 T is the Celsius temperature (°C). d. Tm method: Select a method for determining Tm (DNA melting temperature). Options include: 1st derivative and Average. e. DNA Pair Length (K): Enter the DNA base pair length. If a DNA base pair length is above 5000, enter as 0.
  • Page 28 09931238D Click on the setup button (pencil icon) in the Experiment Setup on the main screen of the software. Setup Single Cell Peltier window will be shown. Set the parameters according to the experiment conditions.  Temperature Control a. Temperature (°C): Enter the preset temperature for the experiment. NOTE: Starting the temperature of experiment needs to be set up in the method window.
  • Page 29 09931238D  Temperature Display: Selected temperature will be displayed on the panel:Block, Probe1 or Probe2. The selected temperature is only displayed in real time.  Option: This function is used to adjust temperature. It is only used for the manufacturing process, so do not modify the values.
  • Page 30 09931238D a. Start Temp (°C): Shows the starting temperature of experiment. b. Measurement Temp (°C): Shows the temperature at which the sample will be measured. c. SP Temp (°C): Shows the current temperature while it goes up/down to the measurement temperature.

  • Page 31: Troubleshooting

    09931238D Troubleshooting Power LED is not lit on Check the connection of the power cable or the fuse. The fuse is located at the rear of the instrument. There is risk of receiving a fatal electric shock if the fuses are replaced with the power cord connected.
  • Page 32 Push the Air Vent Manual button to be switched on and check if coolant flows properly for about one minute after the error occurs. If the Flow Sensor LED is continuously lit on with an alarm sounding, contact your PerkinElmer service representative. Pump LED is lit on with an alarm sound Check whether the tubing is bent or whether it is connected correctly, and then restart the Peltier Controller.