Sample Program - VWR Peqlab Primus 25 advanced Instruction Manual

Instruction manual Primus 25 advanced®

6.2 Sample program

Select MODE, then PROGRAM and create a new program with NEW. Enter a program name and con-
firm with Enter to access the programming functions.
01 LIDHT
Press Enter to insert a new program step. Move from each parameter to the next with the cursor keys
() or with Enter.
02 TEMP
Enter another program step with Enter. Choose one of the steps listed above by using the () cursor
keys, e.g., LOOP[.
03 LOOP[
Continue by entering a temperature profile.
04 TEMP
05 TEMP
06 TEMP
End the cycling step with LOOP] and with the next step switch the heated lid off.
07 LOOP]
08 LIDHT
Ramps may be inserted into any program. The ramp starts from the last programmed temperature (here
72 °C) and decreases temperature to the set temperature (here 8 °C) at a constant rate.
09 RAMP
10 STORE
11 End
PEQLAB_v0314_E
ON 120 °C
94.0 °C +0.0 °C
0:02:00 +0:00
30x
94.0 °C +0.0 °C
0:00:30 +0:00
50.0 °C -0.5 °C
0:00:30 +0:00
72.0 °C +0.0 °C
0:00:30 +0:05
OFF
4.0 °C
1.0 °C/s
8.0°C +0.0°C
Forever +0.00
Heated lid on, temperature 120 °C
Denaturation at 94 °C
For 2 minutes
Open a program loop - 30 cycles
Denaturation at 94°C
For 30 seconds
(Press 0 to enter the time in seconds)
Annealing at 50°C with stepwise
temperature decrement by 0.5 °C (Touch-
down)
Elongation with stepwise time increment
by 5 seconds
End the program loop
Lid heat off
Target temperature 8 °C with
a speed of 1 °C per second
Final temperature 8 °C
(Minus key!)
End of program
7