Programming; Program Steps - VWR Peqlab Primus 25 advanced Instruction Manual

Instruction manual Primus 25 advanced®

6 PROGRAMMING

Thanks to the convenient user interface of the Primus 25 advanced®, it is easy to create PCR programs
of various degrees of complexity.
In the main menu, move the cursor to MODE/EDIT/ UTILS and press Enter twice, or go to the PRO-
GRAM menu with the Mode key.
The following options are available:
NEW To create a completely new program.
EDIT To change an existing program.
COPY To copy a previously stored program (e.g. to modify a program).
DELETE To delete one or all programs.

6.1 Program steps

The following program steps are available for programming the Primus 25 advanced
a) Temperature
TEMP
b) Temperature change within a program loop. The sign states if the temperature is
increased or decreased.
c) Period of time for which the preset temperature is to be held. Using the „minus"
key "FOREVER" can be chosen
d) Time change within a program loop. The sign indicates whether the time is
increased or decreased.
RAMP
The ramp always starts at the temperature defined last and runs to the set temperature.
PAUSE
The process is paused for the stated time at the preset temperature. The pause may be inter-
rupted at any time by pressing a key. The thermal cycler emits an audible signal when the
pause is reached. After pressing the key Enter, the program continues with the next step.
LOOP[
Start of a program loop which is closed by a subsequent Loop] step. Input the number of
cycles. No "loop within a loop" (nested loops) are possible!
LOOP]
End of a program loop.
LIDHT
The heated lid may be switched on or off. The stated temperatures are kept constant within
the program after the Lidheat step. Temperatures from 70 °C to 120 °C are valid.
STORE
Cooling to 8°C, the time is programmable.
LIDOP
The program is stopped and the user is asked to open the lid.
LIDCL
The program is stopped and the user is asked to close the lid.
END
The END step cannot be programmed or deleted, but is always found at the end of a pro-
gram. When reaching this step, the instrument is actively run to ambient temperature and the
operator is called to the instrument .
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