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Lam bda 365 Fiber Coupling M odule and Fiber Optic Probe
This instruction sheet describes the installation of this accessory which is used with the Lambda 365
Spectrophotometer.
NOTE:
Read these instructions before you install this accessory.
Contacting P erk inElm er
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for information:
If you are located within the U.S., call toll free 1-800-762-4002, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
•
Durable stainless steel design
•
Dip Probe for measuring liquid samples without using a cell
Figure 1 Lambda 365 Fiber Coupling Module [Part No: N4101013] with Fiber Optic
Probe [Part No: N4101048]
I nstallation I nstructions
09931262E
PerkinElmer, 710 Bridgeport Avenue,
Shelton, CT 06484-4794, U.S.A
Produced in the USA.
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Summary of Contents for PerkinElmer Lambda 365 Fiber Coupling Module
- Page 1 If you are located within the U.S., call toll free 1-800-762-4002, 8 a.m. to 8 p.m. EST. Your order will be shipped promptly, usually within 24 hours. If you are located outside of the U.S., call your local PerkinElmer sales or service office. Features •...
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Page 2: Dimensions And Specifications
09931262E Dim ensions and Specifications Figure 2 Fiber Optic Probe Figure 3 Fiber Coupling Module Physical Characteristic Specification A: Fiber Type Fused Silica B: Fiber Size 600 µm C: Connector SMA 905 Fiber Optic Probe (Part No: N4101048) D: Probe Diameter ∅... -
Page 3: Installation
09931262E I nstallation 1. Prepare the Lambda 365 Spectrophotometer to install this accessory. 2. Prepare the fiber coupling module and fiber optic probe. 3. Remove the two M3x10L cross recessed flat head screws with washer using a Phillips screw driver. Figure 7 Separate the optical fiber probe clamp 4. - Page 4 09931262E 6. Connect the connectors of the fiber optic prober to the Collimator holder and the SMA port. Figure 10 Connecting the fiber optic probe 7. Make sure that the fiber coupling module and fiber optic probe are connected correctly as below. Figure 11 Fiber coupling module with fiber optic probe 8.
- Page 5 09931262E 9. Pull out the cell holder and base plate by hand. Figure 13 Pulling out the cell holder and base plate 10. Insert the Fiber Coupling Module with fiber optic probe into the sample compartment. Figure 14 Inserting the Fiber coupling module with fiber optic probe 11.
- Page 6 09931262E M easurem ent System Self Test The Fiber coupling module with fiber optic probe should be installed after System Self Test is finished. NOTE: Start the System Self Test after warming up the system for at least 20 minutes. NOTE: 1.
- Page 7 09931262E Scan M ode 1. Install the fiber coupling module with fiber optic probe, referring to the “Installation” procedures on page 3. 2. Select New to open a new window. Select Experiment Type and select OK. 3. Click Method and set up parameters. 4.
- Page 8 09931262E Experiment: SBW is set 2.0 nm automatically. Setup other experiment parameters. Lamp: Single Front is automatically selected. If not, select Single Front and setup other experiment parameters. Lambda 365 UV Setup the Internal Reference, Scan Setup. For more information, see the Express Software Users Guide Scan Setup , the section on...
- Page 9 09931262E Quantification M ode 1. Install the fiber coupling module with fiber optic probe, referring to the “Installation” procedures on page 3. 2. Select New to open a new window. Select Experiment Type and select OK. 3. Select Method and set up the parameters. 4.
- Page 10 09931262E Experiment: SBW is set 2.0 nm automatically. Setup other experiment parameters. Lamp: Single Front is automatically selected. If not, select Single Front and setup other experiment parameters. Lambda 365 UV Express Setup the Quantification parameters. For more information, see the Software Users Guide Quantification , the section on...
- Page 11 09931262E Scanning Quantification M ode 1. Install the fiber coupling module with fiber optic probe, referring to the “Installation” procedures on page 3. 2. Select New to open a new window. Select Experiment Type and select OK. 3. Select Method and set up the parameters. 4.
- Page 12 09931262E Experiment: SBW is set 2.0 nm automatically. Setup other experiment parameters. Lamp: Single Front is automatically selected. If not, select Single Front and setup other experiment parameters. Lambda 365 UV Express Setup the Quantification parameters. For more information see the Software Users Guide Scanning Quantification , the section on...
- Page 13 09931262E K inetics M ode 1. Install the fiber coupling module with fiber optic probe, referring to the “Installation” procedures on page 3. 2. Select New to open a new window. Select Experiment Type and select OK. 3. Select Method and set up parameters. 4.
- Page 14 09931262E Experiment: SBW is set 2.0 nm automatically. Setup other experiment parameters. Lamp: Single Front is automatically selected. If not, select Single Front and setup other experiment parameters. Setup the Kinetics parameters. For more information see the Lambda 365 UV Express Software Kinetics Mode Users Guide, the section 5.
- Page 15 09931262E Scanning K inetics M ode 1. Install the fiber coupling module with fiber optic probe, referring to the “Installation” procedures on page 3. 2. Select New to open a new window. Select Experiment Type and select OK. 3. Select Method and set up parameters. 4.
- Page 16 09931262E Experiment: SBW is set 2.0 nm automatically. Setup other experiment parameters. Lamp: Single Front is automatically selected. If not, select Single Front and setup other experiment parameters. Lambda 365 UV Express Software Setup the Kinetics parameters. For more information see the Users Guide Scanning Kinetics Mode , the section...
- Page 17 09931262E W avelength Program M ode 1. Install the fiber coupling module with fiber optic probe, referring to the “Installation” procedures on page 3. 2. Select New to open a new window. Select Experiment Type and select OK. 3. Select Method and set up parameters. 4.
- Page 18 09931262E Experiment: SBW is set 2.0 nm automatically. Setup other experiment parameters. Lamp: Single Front is automatically selected. If not, select Single Front and setup other experiment parameters. Lambda 365 UV Express Software Users Guide Setup the parameters. See the , the section Wavelength Program Mode 5.
- Page 19 09931262E Alignm ent of Fiber Optic P robe NOTE: When the fiber optic probe is assembled to the fiber coupling module for the first time, or if the alignment is inaccurate, i.e., the intensity is too low or saturated; you need to perform the intensity alignment as described in the following procedures.
- Page 20 09931262E 5. If the baseline measurement has abnormal results, the Fiber Optic Probe has to be aligned. Ex) Intensity is too low. 6. Loosen the three M3 setscrews on the collimator holder using a 1.5 mm wrench. 7. Move slightly back and forth the fiber optic probe connector to the light source side for the alignment, and then tighten the screw on the collimator holder.
- Page 21 09931262E 8. After finishing the alignment, measure baseline again to check suitability. If it is fine, measure the sample. (Maximum intensity should be over 2000 cnt.) If not, repeat the alignment procedure 1 to 7 above.
- Page 22 09931262E...