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PerkinElmer AAnalyst 300 Manual

PerkinElmer AAnalyst 300 Manual

Atomic absorption spectrophotometer

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PerkinElmer AAnalyst 300
Location: Friedland 202
PerkinElmerAA300.jpg:
How It Works and What It Does
The AA-300 is used to determine the unknown concentration of an individual element by measuring the amount of light the unknown
concentration absorbs. Instrument readouts are calibrated to display concentration directly. The basic instrumentation requires a primary light
source, an atom source and a detector. The light source is a hollow cathode lamp (HCL) or electrode discharge lamp (EDL), which emits a
spectrum specific to the element from which it is made. The light beam passes through either an air-acetylene or nitrous oxide-acetylene flame,
which has a high temperature and specific flow rate. The atom source introduces the sample into the flame as an aerosol and the heat produces
free analyte atoms. Analyte atoms in the flame that correspond to the particular lamp being used absorb energy from the light source. A detector,
usually a photomultiplier tube, measures the light intensity exiting the flame. The electronics convert the amount of light absorbed to the actual
sample concentration.
The AA-300 is equipped with an automatic six-lamp turret and is programmed to perform sequential multielement analysis. The AA-300 is
equipped with lamps for the following elements: Ag, Al, As, Ba, Ca, Cd, Cr, Cu, Fe, K, Na, Mg, Ni, Pb and Zn The Iron(Fe) and Copper(Cu) lamps
are the most commonly used.

Directions for Use

Instructions for Use
by Golda Yelaun
Instruction for AA – Flame Analyst-300.
1.
Check the location of exhaust vent (should be located at least 4 meters from the Flame).
2.
Check the pressure of the AA Gas Purifier assembly. Make sure the output pressure is between 51 and 65 psig. ( on the wall , behind the
instrument).
3.

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Summary of Contents for PerkinElmer AAnalyst 300

  • Page 1: Directions For Use

    PerkinElmer AAnalyst 300 Location: Friedland 202 PerkinElmerAA300.jpg: How It Works and What It Does The AA-300 is used to determine the unknown concentration of an individual element by measuring the amount of light the unknown concentration absorbs. Instrument readouts are calibrated to display concentration directly. The basic instrumentation requires a primary light source, an atom source and a detector.
  • Page 2: Instrument Calibration

    Turn on the proper gas supplies and set pressures, recommended in the handbook. Acetylene 85-100 kPA (12-14 psig) Air (dry grade) 350-450 kPA (50-65 psig) Nitrous oxide 345-520 kpa (50-75 psig) Switch on the computer and start the Windows. Switch on the spectrometer and accessories. Start the AAWinLab.
  • Page 3 repeat the calibration. Example: Creating a Calibration Curve for Fe (Iron) by Golda Yelaun 1. Creating a Calibration Curve Preparing the calibration standards. Calibration standards are prepared from stock solution 1000 PPM Fe (Atomic Absorption standard). We prepared three calibrations standards: 2ppm, 5ppm, 10ppm. In the Manual Analysis Control window we have to click Analyze Blank, then Analyze Standard.Since the Method is already stored all three standards (2,5,10 ppm) will be analyzed.
  • Page 4 Contact Prof. Dolnik or Golda Yelaun for more information. Click here for departmental directory Main Users of Instrument The main users are General and Honors General Chemistry (Chem 18 and 19). Occasionally other advanced chemistry courses use it too. Web Resources Misc AA300 PerkinElmer info...