Available Trapping Columns; Available Formats - Thermo Scientific UltiMate 3000 Standard Applications Manual

2.2

Available Trapping Columns

2.2.1

Available Formats

UltiMate 3000 RSLCnano Standard Applications Guide
Trap columns are available in two formats, which are a cartridge-based
µ-precolumn (Figure 5) and a nano trap column
trapping columns are UHPLC compatible due to the nanoViper fittings
employed. The choice between a µ-precolumn or a nano trap depends
on application needs such as flexibility, sample loading flow rate and
robustness as well as sample quality, desired loading capacity and
personal preference.
Figure 5: µ-Precolumn (cartridge-based)
TIP P/N 164648 contains two 30 µm ID x 100 mm nanoViper capillaries
and can be used to order replacement capillaries
µ-precolumns are small trap cartridges that are inserted into a cartridge
holder, connected to the switching valve by two 30 µm ID x 100mm
nanoViper capillaries. The stationary phase is retained by a frit at both
ends of the cartridge allowing the mobile phase to flow through it in
both directions without disrupting the column packing. Therefore,
µ-precolumns can be used in both forward- and back-flush operation
(see section 2.2.2 for details). The bed volume is large, but short, giving
it higher absolute loadability compared to nano traps, but the short bed
could result in earlier sample breakthrough for hydrophilic components.
Backpressure is lower compared to nano trap columns and therefore µ-
precolumns can accommodate higher loading flows and are often
preferred when large sample volumes need to be injected.

2 Application Setup
(Figure 6). Both types of
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