Flow Meter Calibration - Thermo Scientific UltiMate 3000 Standard Applications Manual

2.3.5

Flow meter calibration

ProFlow flow meter solvent calibration
2.3.5.1
Viscosity Measurement Test using the Classic Flow Meter
2.3.5.2
UltiMate 3000 RSLCnano Standard Applications Guide
Solvent calibration routines differ for the two types of flow meter.
The ProFlow flow meter uses thermal flow sensors for direct
measurement of solvent flow. The ProFlow flow meter comes pre-
calibrated with four solvent types: Water, 100% Acetonitrile, 80%
Acetonitrile and 100% Methanol. The calibration values for these solvent
types are stored locally on the flow meter and are valid for the life of the
flow meter. They are also valid when the flow meter is transferred from
one UltiMate 3000 RSLCnano system to another.
NOTICE The solvent types are valid for a variation in composition ≤ 2%
i.e. a solvent consisting of 98% Water / 2% Acetonitrile can be run using
the pre-calibrated solvent type Water. This is also applicable if the added
modifier changes >2%.
For all other solvents, a custom solvent calibration is required.
Custom solvent calibrations are only valid for the channel on which they
are carried out. To perform a custom solvent calibration, click on the
"Wellness" button on the NC_Pump panel and then"Calibrate Solvent".
A wizard guides the user through the calibration procedure.
TIP Custom solvent calibrations are also stored locally on the flow meter
hardware and are valid for the life of the flow meter.
The solvent type for the A and B channels should match the solvents
used in the application. To select the solvent type, go to the
PumpModule tab of the ePanel. Under NC Pump -> More Options select
the desired solvent type for each channel from the drop down menu
under 'solvents'.
The classic flow meter uses the pressure drop across a restriction
capillary (integrated in the flow selector) to determine solvent flow rate.
During the viscosity measurement calibration, the resistance of the
solvent is measured relative to the factory calibration value for water
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2 Application Setup
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