Customized Sample Injection Routines; Introduction To User Defined Program (Udp) Injection Routines; Important Considerations When Writing A Udp; The Udp Commands - Thermo Scientific UltiMate 3000 Standard Applications Manual

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4 Appendix
4.1

Customized Sample Injection Routines

4.1.1

Introduction to User Defined Program (UDP) Injection Routines

4.1.2

Important Considerations when Writing a UDP

4.1.3

The UDP Commands

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WPS-3000TPL RS autosamplers are equipped with standard injection
routines for Full-loop, Partial-loop and µL pickup, which are suitable for
the majority of applications. However, for applications requiring special
injection routines, or for users wishing to customize one of the standard
injection methods, a UDP should be used.
UDPs provide the user with full control over every aspect of the injection
routine and enable every type of injection, from simply filling the loop
and injecting the sample to complete liquid handling, such as in-well
digestion.
Although a UDP is flexible, it is also unforgiving: every step of the
injection routine must be programmed manually and missing steps will
lead to erroneous injections. The Chromeleon / SII software will execute
the steps sequentially until it encounters an error after which it will
either continue to the next sample or interrupt the analysis.
The general layout of a UDP can be broken down into five different
steps:
1. Sample preparation (only when applying liquid handling)
2. Preflush of the injection needle
3. Filling the sample loop
4. Injecting the sample and starting the acquisition run
5. Washing the syringe and preparing for the next injection
Step '1' is often omitted, as samples are usually ready to inject when
placed in the autosampler.
UltiMate 3000 RSLCnano Standard Applications Guide