Installing And Configuring The Application Fluidics; Interfacing The Ultimate 3000 Rslcnano With The Nanospray Flex™ Ion Source - Thermo Scientific UltiMate 3000 Standard Applications Manual

2.1.3

Installing and Configuring the Application Fluidics

2.1.4 Interfacing the UltiMate 3000 RSLCnano with the Nanospray Flex™ Ion
Source
UltiMate 3000 RSLCnano Standard Applications Guide
3. Tighten both sides of the black nut equally to ensure that the nano
connector sleeve is in the center of the transparent union
4c.
a
b
c
Figure 4: Nano connector assembly. (a) The two black nuts and
transparent union are mounted on the two outlets to be connected.
(b) A dead volume free connection with the nano connector. (c) The
complete fitting with the black nuts and union housing the nano
connector.
Each capillary must be installed sequentially starting from the pump
outlet. Please flush each capillary using the respective pump and ensure
that a droplet is visible at the capillary outlet in question before making
the next connection. This will ensure that all air is removed from the
capillaries and connections and that no air is passed through the
column.
LC−MS based applications using linear columns commonly use the
Nanospray Flex Ion source (see
Scientific Mass Spectrometers.
(20 µm and 50 µm) and a tile for cutting fused silica capillaries have
been included in the pump (NCS-3500RS and NCP-3200RS) accessory
kits. This capillary should be used to connect the outlet of the column or
UV flow cell, if included in the setup, with the emitter installed in the ion
source. The connection between the capillary outlet and the emitter is
realized using a 1/32" micro tight® union assembly included with the
Nanospray Flex Ion Source. For capillaries and columns with 280 µm O.D.
a black sleeve (P/N SC903) should be used with the microtight fitting.

2
Figure 41) to interface with Thermo
As of January 2019, 1.5 m of fused silica
Application Setup
Figure
Page 17