Making Connections Using The Nano Connector - Thermo Scientific UltiMate 3000 Standard Applications Manual


2 Application Setup
2.1.2

Making Connections using the Nano Connector

Page 16
The outlet of the linear nano columns are fitted with a nano connector.
This zero dead volume connection is designed to interface the linear
column outlet with 280 µm fused silica capillaries and is pressure stabile
up to 300 bars. The nano connector uses a special sleeve to ensure
pressure tightness. The assembly of a nano connector is described step
by step in Figure 2 below.
Figure 2: The components of the nano connector

Use a new nano connector sleeve (P/N 6720.0391) each time the
connection is made.
NOTICE: Do NOT use a PTFE sleeve (P/N 160486; supplied with the
columns). The size does not match the nano connector union
and the pressure resistance is much lower.
Figure 3: Nano connector (top) and PTFE (bottom) sleeve comparison
1. Slide the black nut and transparent union onto one of the ends of
the fused silica, and the other black nut onto the other fused silica
end Figure 4a.
2. Slide the nano connector sleeve onto one end of the fused silica
until it reaches the middle of the sleeve. Slide the other end of the
fused silica into the connector sleeve. Make sure that the
connection is dead volume free (i.e. that the ends meet in the
middle Figure 4b).
UltiMate 3000 RSLCnano Standard Applications Guide
Nano connector sleeve (1.1 cm)
PTFE sleeve (1.8 cm)
(Figure 3)