In Vivo Delivery To Epidermis - BIO RAD Helios Gene Gun System Instruction Manual

In Vivo Delivery to Epidermis

Animal Preparation
1. Anesthetize the animal if necessary for safe handling.
2. Clip fur as closely as possible over the desired target area using Oster clippers with a #40 surgical blade
and brush or vacuum fur off.
3. (Optional) After clipping, a commercial depilatory such as Nair can be used to completely remove the
animal's fur. This treatment removes the stratum corneum from the skin, completely exposing the
epidermis. Carefully rinse the skin with warm water following depilatory treatment.
4. If the target site is wet or dirty, clean and dry with 70% ethanol.
Helios Gene Gun Operation
1. Prepare the Helios Gene Gun for operation as described in Section 5.3.
2. Hold the spacer directly against the target site (Figure 19) and discharge the device. (Discomfort
following Helios Gene Gun bombardment of the skin is minimal. No apparent macroscopic disruption
of the skin, external bleeding or hematoma should be observed. Some animals may show transient
erythema or inflammation at the treatment site.)
Fig. 19. In vivo bombardment of murine epidermis (photo courtesy of Auragen, Inc.).
Section 6 Preparation of Mammalian Cell Targets
Helios Gene Gun System | 35