BIO RAD Helios Gene Gun System Instruction Manual page 41

Fig. 20. Luciferase expression in mouse skin transfected in vivo using the Helios Gene Gun. Skin homogenates were assayed 24 hours
posttransfection.
As illustrated in Figure 21, the optimum MLQ is determined by adjusting the concentration of the DNA/
microcarrier suspension loaded into the GoldCoat Tubing. For suspension cells, higher MLQs (0.5–1.0 mg
gold/cartridge) are often needed due to the high cell concentrations in the aqueous cell smear. Adherent cell
monolayers and intact tissues may require reduced MLQs (0.06–0.25 mg gold/cartridge) to minimize tissue
damage while maximizing transfection.
Fig. 21. Effect of the amount of accelerated gold particles on in vitro expression levels. Suspension cultures of CHO cells were bombarded
as described in Section 6.2 using the Accell Gene Gun. After 18 hours, the CHO cells that had reattached to the tissue culture plate were lysed and
assayed for luciferase expression.
80
60
40
20
0
0 100 200
Helium pressure, psi
3.0
2.5
2.0
1.5
1.0
0.5
0
1.0 0.5 0.25
mg gold per cartridge
Section 7 Optimization of Gene Gun Parameters
400
0.12 0.06
Helios Gene Gun System | 37