Quantification Of Dna In Cartridges - BIO RAD Helios Gene Gun System Instruction Manual

Section 10 Appendices

Quantification of DNA in Cartridges

This procedure can be used for estimating the amount of DNA in cartridges when 0.5 µg or more of DNA
has been loaded per tube.
Materials
10 mM Tris, pH 8.0, 1 mM EDTA
■
1,000 µ micropipettor and tips
■
Ultrasonic cleaner
■
UV spectrophotometer
■
Vortexer
■
Quartz cuvettes
■
Microfuge
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Procedure
1. Place five 0.5" cartridges in a microcentrifuge tube and add 500 µl of TE.
2. Sonicate and/or vortex to dislodge gold and solubilize the DNA. Spin for 5 min in a microfuge to
pellet gold.
3. On a spectrophotometer, determine the A
precipitated onto the microcarriers, the A
Plasmid/Cartridge, µg A
0.5 0.1
1.0 0.2
2.0 0.4
4. DNA sample may also be analyzed by restriction enzyme digestion and analysis by agarose
gel electrophoresis.
50 | Helios Gene Gun System
by reading against a TE blank. If 100% of the DNA were
260
readings should be as follows:
260
260