Setting Up The Tubing Prep Station - BIO RAD Helios Gene Gun System Instruction Manual

Section 4 Inserting the Battery into the Helios Gene Gun
2. In a similar manner, insert the stem of the Swagelok Quick-Connect Fitting on the Helios Gene Gun into
the opening in the body of the Swagelok Quick-Connect Fitting on the helium hose until it clicks. The
gene gun will be locked into the helium hose.
Sleeve
Stem
Fig. 10. Connecting the helium hose to the helium regulator.

Setting up the Tubing Prep Station

Refer to Identification of System Components and Controls, in Section 3, prior to system installation. See
Figure 11 for a diagram of the assembled Tubing Prep Station.
The Tubing Prep Station is shipped disassembled. The following sections describe assembly of the Tubing
Prep Station, attachment of the syringes and tubing, installation of the nitrogen pressure regulator, and
connection of the Tubing Prep Station to the nitrogen regulator.
A peristaltic pump is recommended for removal of the ethanol from the GoldCoat Tubing after the
microcarriers have been loaded (see Section 5.1). If a peristaltic pump is not available, you may remove the
ethanol manually using a syringe. Assembly of the syringe and tubing is described in step 5 of the following
section.
Assembly of the Tubing Prep Station and Syringes
Components needed
Tubing Prep Station, base
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Tubing Prep Station, tubing support cylinder
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Tubing Prep Station, power cord
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O-rings, for tubing support cylinder
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Syringe adapter tubing (silicone, 5', 0.104" ID, 0.192" OD)
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16 | Helios Gene Gun System
Connection to helium tank
Helium regulator
Pressure relief valve
Female Swagelok Quick-Connect
Fitting with Check Valve
Male Swagelok Quick-Connect Fitting
Helium hose (6')
Connection to gene gun
Female Swagelok Quick-Connect Fitting