BIO RAD Helios Gene Gun System Instruction Manual page 11

Battery
One battery is provided with the Helios Gene Gun System. Under normal use, it should provide
approximately 1,000 discharges. For maximum life, use only alkaline batteries.
Laboratory Equipment
The following materials should be available before beginning any work with the Helios System.
Ultrasonic cleaner (for example, Fisher FS3, Branson 1210)
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Vortex mixer
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Analytical balance
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Microcentrifuge
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Peristaltic pump capable of pumping 5–8 ml/min
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1.5 ml microcentrifuge tubes
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20, 200, and 1,000 µl micropipettors and tips
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5 and 10 ml pipets and pipettors
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Lab timer
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Ear protection (for example, VWR catalog number 56610-728 (earmuffs) or 56610-680 (ear plugs)
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One 1/8" open end or 10" or 12" (~25 cm) adjustable wrench
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Helium tank (grade 4.5 or higher)
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Nitrogen tank (grade 4.8 or higher)
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Nitrogen regulator (for example, Bio-Rad, #1652425)
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Scissors
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Marking pen
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Laboratory reagents
The following chemicals will be needed for coating plasmid onto the gold and for preparing the tubing:
Gold microcarriers
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Polyvinylpyrrolidone, MW = 360,000
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100% ethanol (for example, Spectrum Chemical, catalog number ET-107; it is extremely important that
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this be free of water; an unopened bottle should be used daily)
Section 2 Introduction to Particle Delivery
Helios Gene Gun System | 7