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Omni Bead Ruptor 24 User Manual

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Summary of Contents for Omni Bead Ruptor 24

  • Page 2: Table Of Contents

    SECTION 1 IMPORTANT SAFEGUARDS ........................4 SECTION 2 UNPACKING THE BEAD RUPTOR 24 2.1 Unpacking The Bead Ruptor 24 ............................5-6 2.2 Unit Overview ....................................7 2.3 Components ....................................8 2.4 Additional Accessories ................................8-9 SECTION 3 BEAD RUPTOR SAMPLE TUBES 3.1 Tubes &...

  • Page 3: Warranty Information

    WARRANTY INFORMATION This manual is a guide for the use of the Bead Ruptor 24 Bead Mill Homogenizing System and accessories. Data herein has been verified and validated. It is believed adequate for the intended use of the instrument. If the instrument or procedures are used for purposes over and above the capabilities specified herein, confirmation of the validity and suitability should be obtained, otherwise Omni International does not guarantee results and assumes no obligation or liability.

  • Page 4: Section 1 - Important Safeguards

    Do not attempt to modify any part of the Bead Ruptor 24. If you experience problems with or have questions about your Bead Ruptor 24, contact your authorized dealer or call Omni at 1-800-776-4431 or 770-421-0058.

  • Page 5: Section 2 - Unpacking The Bead Ruptor

    2.1 UNPACKING THE BEAD RUPTOR 24 CAUTION: DO NOT lift the Bead Ruptor 24 by holding the lid. It must be lifted by gripping the sides of the unit and holding it from the bottom. 1. Place the crate on a level floor surface.
  • Page 6 • DO NOT turn the unit upside down: the Bead Ruptor 24 must always rest on all four feet to avoid damaging internal components or breaking the housing. • DO NOT operate the unit when the housing is removed;...

  • Page 7: Unpacking The Bead Ruptor 24

    SECTION 2 — UNPACKING THE BEAD RUPTOR 2.2 UNIT OVERVIEW Keypad Lid Latch Finger Plate Lock Switch Tube Carriage Finger Plate 120V/220V Switch & Fuse ON/Off Switch Shipping Screw Holes AC Power Input...

  • Page 8: Components

    SECTION 2 — UNPACKING THE BEAD RUPTOR 2.3 COMPONENTS Prior to operation, please remove all parts from the shipping container and inspect for damaged or missing parts. If any parts are found to be damaged or mising, please contact Omni International at 800.776.4431. DESCRIPTION QUANTITY...
  • Page 9 Works exclusively with the Bead Ruptor 24. The BR-Cryo 24 is not compatible with 15ml, 30ml and the 50ml tube carriages...

  • Page 10: Bead Ruptor Sample Tubes

    SECTION 3 — BEAD RUPTOR SAMPLE TUBES 3.1 TUBES & TUBE CARRIAGES 96 x 1.2 mL strip well tubes 1.2 mL Strip Well volume and tissue size Minimum: 200 µL, 10 mg Maximum: 1.0 mL, up to 750 mg CAUTION: The maximum recommended speed for the 1.2 mL strip well tube carriage is 5 m/s.

  • Page 11: Tubes & Tube Carriage

    The maximum recommended speed for the 50 mL Tube Carriage is 5 m/s. The 50ml tube carriage is not compatible with the BR-Cryo lid CAUTION: Use only Omni sample tubes. Accuracy and operating lifetime may be affected if alternate tubes are used.

  • Page 12: Ordering Information

    SECTION 3 — BEAD RUPTOR SAMPLE TUBES SECTION 3 — BEAD RUPTOR SAMPLE TUBES 3.2 ORDERING INFORMATION 1.5 mL PRE-FILLED BEAD MILL MICROTUBES TUBE BEAD DNAse/RNAse BEAD MEDIA DESCRIPTION QUANTITY SIZE SIZE FREE PART# Soft Tissue Ceramic 1.5 mL 1.4 mm 19-617* Homogenizing Mix Tough Micro-organism...
  • Page 13 SECTION 3 — BEAD RUPTOR SAMPLE TUBES 3.2 ORDERING INFORMATION 2 mL & 0.5 mL SCREW CAP PRE-FILLED BEAD MILL TUBES TUBE BEAD DNase/RNase BEAD MEDIA DESCRIPTION QUANTITY SIZE SIZE FREE PART# Micro-Organism 2 mL 0.1 mm 19-621 Lysing Mix Glass Tough Micro-Organism 2 mL...
  • Page 14 SECTION 3 — BEAD RUPTOR SAMPLE TUBES SECTION 3 — BEAD RUPTOR SAMPLE TUBES 3.2 ORDERING INFORMATION (cont.) 7 mL PRE-FILLED BEAD MILL TUBES BEAD MEDIA DESCRIPTION TUBE SIZE BEAD SIZE QUANTITY ORDER NUMBER Metal 7 mL 2.38 mm Hard Tissue Grinding Mix 19-670 Soft Tissue Homogenizing 7 mL...
  • Page 15 SECTION 3 — BEAD RUPTOR SAMPLE TUBES 3.2 ORDERING INFORMATION (cont.) 30 mL PRE-FILLED BEAD MILL TUBES BEAD MEDIA DESCRIPTION TUBE SIZE BEAD SIZE QUANTITY ORDER NUMBER 30 mL 2.38 mm Hard Tissue Grinding Mix 19-6350 Metal Hard Tissue Impaction Grinding 30 mL 2.38 mm 19-6350Z...

  • Page 16: Important Sample Tube Information

    Bead Ruptor sample tubes. The intratube forces generated by the Bead Ruptor 24 during the homogenization process are up to 400 x g. Intratube forces are dependent on the speed setting and the bead media present inside the tube. Omni pro- vides unique tubes that are designed to withstand such high forces.

  • Page 17: Loading Sample Tubes

    SECTION 4 — BEAD RUPTOR OPERATION 4.1 LOADING SAMPLE TUBES When using a 1.5 mL microtube carriage or 2 mL Tube Carriage, a minimum of 4 tubes (filled or empty) are required per run and must be spaced evenly. The positions of 1, 7, 13, and 19 on the 1.5ml and 2mL tube carriage are ideal for balancing the tubes (refer to the numbers etched into the tube carriage).
  • Page 18 SECTION 4 — BEAD RUPTOR OPERATION 4.1 LOADING SAMPLE TUBES (cont.) CAUTION: The drive hub can become very hot during operation. Use caution when removing tubes from the Tube Carriage / Tube Holders. IDENTIFICATION OF PARTS: 7 mL Tube Carriage Lock Ring 1.
  • Page 19 SECTION 4 — BEAD RUPTOR OPERATION 4.1 LOADING SAMPLE TUBES (cont.) 30 mL Tube Carriage 1. Place Bead Ruptor 30 mL tubes into the Tube Carriage. 2. Place the tube carriage onto the Drive Hub. Lock Ring 3. Screw the locking ring on tightly. 4.

  • Page 20: Keypad Layout

    4.3 START UP OF THE EQUIPMENT Turn the Bead Ruptor 24 on by pressing the ON/OFF switch located on the rear panel near the AC power input. When the unit is turned on, a welcome screen is displayed for five seconds showing the following information:...

  • Page 21: Speed Setting

    Bead Ruptor tubes. The rate of collision and energy of impact determine the effectiveness of the disrup- tion process and are controlled by the Bead Ruptor 24 “S” setting, the motion of the tube carriage, the bead media, and the tube fill volume.

  • Page 22: Programming

    • Homogenization settings cannot be changed once the run is in progress • The Bead Ruptor 24 is designed for non-stop use and does not require a cool down period between runs. The user can pause or stop a run by pressing the “STOP” button at any time. The unit displays the Cancel...

  • Page 23: Adjusting Parameters

    SECTION 5 — PROGRAMMING 5.2 ADJUSTING PARAMETERS Use the  and  Arrow Keys to move the ‘active’ character (or cursor) between the different variables within the program while the  and  buttons respectively decrease or increase the value of whichever variable is active.

  • Page 24: Adjusting Parameters

    SECTION 5 — PROGRAMMING 5.2 ADJUSTING PARAMETERS ADJUSTING THE NUMBER OF CYCLES Use the  and  arrow keys to highlight the “C”- Cycles option. 20 o C PROG 04 S =4.50 C=02 T=0:10 D=0:10 NUMBER OF CYCLES The user can change the Number of Cycles using the  and  Arrow buttons. The number of cycles ranges from 1 to 10.

  • Page 25: Cleaning The Bead Ruptor 24

    SECTION 6 — MAINTENANCE 6.1 CLEANING THE BEAD RUPTOR 24 The housing of the unit can be cleaned with a sponge or damp cloth moistened with water or alcohol. WARNING: For safety purposes and to prevent any damage of the unit, the recommendations listed below should be strictly followed: • DO NOT...

  • Page 26: Troubleshooting

    SECTION 7 — TROUBLESHOOTING DO NOT attempt to service the Bead Ruptor 24 in a manner other than those discussed in this manual. For any issue that is unsuccessfully corrected using this guide, please contact your authorized dealer or call Omni International at: 800.776.4431.

  • Page 27: Transport, Storage & Service

    6. Use a screwdriver or power drill to secure the lid to the top of the crate. PLEASE NOTE: The Bead Ruptor 24 MUST be shipped in its original packaging. Failure to do so can result in serious damage to the unit and will void all warranties.

  • Page 28: Storage

    0° C/32° F to 50° C/122° F 8.3 SERVICE It is recommended that the speed of the Bead Ruptor 24 be calibrated annually to ensure maximum operation. For this or any other product servicing please contact Omni International at 800.776.4431.

  • Page 29: Section 9 - Specifications

    Keep your sensitive samples cold! The Cryo 24 is the perfect companion to the Bead Ruptor 24. It keeps samples cool before, after & during processing. Used with liquid nitrogen or dry ice, it maintains a consistent temperature for heat sensitive samples.
  • Page 30 NOTES:...
  • Page 31 NOTES:...

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