Molecular Weight Assignment - GE Amersham Imager 600 Operating Instructions Manual

5.8.4

Molecular weight assignment

Description
This is the fourth step in the analysis workflow. The selected image is displayed on the
screen and the button MW is highlighted in the workflow. The options for molecular
weight assignment are presented beside the image.
Molecular weight is calculated using standard molecular weight (MW) markers.
Assign molecular weight
The following illustration and table below describe how to assign molecular weight.
Note:
Step
1
2
3
Amersham Imager 600 Operating Instructions 29-0645-17 AD
Molecular weight calibration can only be performed in single channel view.
Action
Select the lane containing the molecular weight marker.
Result: The selected lane appears highlighted.
Select the standard molecular weight marker from the drop down menu.
If the correct marker is missing:
Add a missing marker by selecting Add new... button. See instructions
•
below.
Edit a marker by selecting the Edit button. See instruction below.
•
Deselect any missing bands in the sample marker lane by unchecking rele-
vant check boxes.
5 Operation
5.8 Analyze the image
5.8.4 Molecular weight assignment
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