Normalization - GE Amersham Imager 600 Operating Instructions Manual

5 Operation
5.8 Analyze the image

5.8.5 Normalization

5.8.5 Normalization
Description
This is the fifth step in the analysis workflow. The selected image is displayed on screen
and the button Normalization is highlighted in the workflow.
Normalization calculates band volumes relative to one or more reference bands. Select
reference band(s) in the lane and all other bands in that lane will be normalized against
the selected band(s). If multiple reference bands are selected, the reference value is set
as the average of the selected band volumes.
Normalization is useful when comparing relative band volumes in different lanes.
The options for normalization are presented next to the image.
Normalize bands
Follow the instructions below to perform normalization.
Step
1
2
174
Action
Select one or two control bands in a lane.
Result: The selected band(s) appear highlighted.
Note:
Control bands can only be selected in single channel view.
When you are done select the Next button or select the Summary button
in the workflow. The Previous button will bring you back to the previous step.
Amersham Imager 600 Operating Instructions 29-0645-17 AD