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6
– Applications
6. Applications
6.1 Hydrophilic Pharmaceuticals
250
0
0
6.2 Aspirin and Degradation Product
200
mAU
0
0
Thermo Scientific
065339-02
2
1
5
3
4
3
6
9
Minutes
1
2
2
4
6
Minutes
Product Manual for Acclaim HILIC-10 Columns
Column:
Acclaim HILIC-10, 3 µm
Dimensions:
4.6 x 150 mm
Mobile Phase:
90/10 v/v CH
NH
OAc, pH5
4
Temperature:
30 °C
Flow Rate:
1 mL/min
Inj. Volume:
2 µL
Detection:
UV at 230 nm
Peaks:
1. Acetaminophen
2. Salicylic acid
3. Aspirin
4. Penicillin G
5. Metformin
12
-
Column:
Acclaim HILIC-10, 3 µm
Dimensions:
4.6 x 150 mm
Mobile Phase: 90/10 v/v CH
Temperature: 30 °C
Flow Rate:
1 mL/min
Inj. Volume:
1 µL
Detection:
UV at 220 nm
Peaks:
1. Salicylic acid
2. Aspirin
8
10
CN/10 mM (total)
3
mg/mL
0.1
0.1
0.2
0.1
0.1
CN/10 mM (total) NH
OAc, pH5
3
4
mg/mL
0.1
0.2
Page 16 of 23
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