Applications Problems - Thermo Scientific Nicolet iS50 User Manual

Troubleshooting

Applications Problems

This section provides simple troubleshooting measures you can take to solve data collection
problems with the spectrometer. If the action does not solve the problem,
Problem
Spectrum
contains
derivative-
shaped peaks.
Spectrum
contains totally
absorbing
peaks.
Thermo Scientific
Possible Cause
Sample in the diffuse reflection
experiment also has a specular
reflection component.
Infrared beam was reflected from
(instead of penetrating) a flat,
shiny sample measured using a
specular reflection accessory.
An ATR spectrum was attempted
on an extremely strong absorber
or scatterer, such as carbon
black rubber.
Sample in the transmission
experiment is too thick.
contact
Solution
Correct the spectrum by using Apply
Kramers-Kronig Correction in the
Process menu.
Try using a less reflective sample.
Try a Ge crystal on the ATR, if
available. This decreases the depth
of penetration.
Decrease the pathlength by using a
thinner sample.
Nicolet iS50 Spectrometer User Guide
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