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074259
Thermo Scientific 074259 Manuals
Manuals and User Guides for Thermo Scientific 074259. We have
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Thermo Scientific 074259 manual available for free PDF download: Product Manual
Thermo Scientific 074259 Product Manual (23 pages)
Analytical Column
Brand:
Thermo Scientific
| Category:
Laboratory Equipment
| Size: 0.37 MB
Table of Contents
Table of Contents
5
1 Introduction
7
The Main Features of the Acclaim HILIC-10 Columns Include
7
Physical Data
8
Specifications and Recommended Operational Parameters
8
2 Step-By-Step User Guide
9
Step 1 - Visually Inspect the Column
9
Step 2 - Mobile Phase Preparation
9
Step 3 - Set up the LC System
10
Step 4 - Condition the Column
10
Step 5 - Reproduce the Chromatogram in the Quality Assurance Report
10
Step 6 - Real Sample Analysis
10
3 Considerations in Method Development
11
Selection of Organic Solvents
11
Buffer Types
11
Mobile Phase Ph
11
Isocratic or Gradient Method
11
Injection Volume
11
4 Column Care
12
Column Storage
12
Operating Ph Range: Ph 2.0 to 8.0
12
Operating Temperature Limit: 70 ºC
12
Pressure Limit
12
Flow Rate
12
Column Washing Procedure
12
5 Frequently Asked Questions
14
What Is the Difference between the Acclaim HILIC-10 Column and Other HILIC Columns
14
Why Do I Need an Acclaim HILIC-10 Column
14
When Do I Need an Acclaim HILIC-10 Column
14
What Factors Should I Consider for Method Development Using this Column
14
What Mobile Phases Should I Use with this Column
14
What Should I Do before Starting to Use the Acclaim HILIC-10 Column
15
Can I Use this Column in Normal-Phase Mode
15
How to Store the Column
15
6 Applications
16
Hydrophilic Pharmaceuticals
16
Aspirin and Degradation Product
16
Melamine and Cyanuric Acid
17
Solvent Effect of Melamine
17
Carbohydrates
18
Sugar Alcohols
18
Acrylamide and Acrylic Acid
19
Methacrylic Acid and Acrylic Acid
19
Acrylic Acid and Oligomers
20
Good's Buffer Salts
20
Sodium Chloride
21
Tris•Hcl
21
Α-Tocopherol and Α-Tocopherol Acetate
22
Vitamins K1 and K2
22
Retinol Palmitate and Retinol Acetate
23
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