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6
– Applications
6.13 α-Tocopherol and α-Tocopherol Acetate
15
mAU
0
0
6.14 Vitamins K1 and K2
150
mAU
0
0
Thermo Scientific
065339-02
1
2
2
4
6
Minutes
Column:
Dimensions:
Mobile Phase:
1 2
Flow Rate:
Temperature:
Inj. Volume:
Detection:
Peaks:
1
2
3
4
Minutes
Product Manual for Acclaim HILIC-10 Columns
Column:
Acclaim HILIC-10, 3 µm
Dimensions:
4.6 x 150 mm
Mobile Phase:
94.75/5.00/0.25 v/v/v
Heptane:Tetrahydrofuran:Isopropanol
Flow Rate:
1 mL/min
Temperature:
30 °C
Inj. Volume:
5 µL
Detection:
UVD-340U; UV 285 nm
Peaks:
1. α-Tocopherol acetate
2. α-Tocopherol
8
Acclaim HILIC-10, 3 µm
4.6 x 150 mm
94.75/5.00/0.25 v/v/v
Heptane:Tetrahydrofuran:Isopropanol
1 mL/min
30 °C
5 µL
UVD-340U; UV 265 nm; spectra 200-400 nm
µg/mL
1. Vitamin K1
50
2. Vitamin K2
50
5
µg/mL
60
40
Page 22 of 23
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