Thermo Scientific Acclaim HILIC-10 Product Manual page 13

4 – Column Care
1. Wash the column with iso-propanol for 10 column volumes at 20% of the typical flow
2. Wash the column with D.I water /acetonitrile v/v 20/80 for 20 column volumes at 50%
3. Wash the column with iso-propanol for 10 column volumes at 20% of the typical flow
4. Before any injection is made, the column should be equilibrated with the mobile phase
For a 4.6 mm i.d. column, the typical flow rate for washing is 0.8 mL/min.
!
For a 2.1 mm i.d. column, the typical flow rate for washing is 0.15 mL/min.
For a 3.0 mm i.d. column, the typical flow rate for washing is 0.3 mL/min.
NOTE
If above treatment fails to revive the column, the column should be replaced.
Thermo Scientific
065339-02
rate for the column dimension Wash the column with 0.1 M ammonium acetate pH 4 to
5 /acetonitrile v/v 50/50 for 20 column volumes at 50% of the typical flow rate for the
column dimension.
of the typical flow rate for the column dimension.
rate for the column dimension.
thoroughly.
Product Manual for Acclaim HILIC-10 Columns Page 13 of 23