Calibrate The Objectives - Thermo Scientific EVOS M5000 User Manual

Calibrate the objectives

Calibrate objective
magnification
62
Calibrate objective magnification allows the calibration of the field of view,
parfocality, and parcentration parameters of the selected objective. When
calibrated, parfocality ensures that the sample stays in focus when the objective is
changed, and parcentration ensures that an object in the center of the field of view
stay in the center of the field no matter which objective is being used.
Note: The pre-installed objectives supplied with the EVOS
System have been pre-calibrated. You do not need to calibrate them again unless
they are reinstalled after removal from the instrument.
Calibration procedure requires the use of the EVOS
supplied with the EVOS
time required objective calibration is about 5 minutes.
Note: For best parfocality and parcentration, calibrate the installed objectives one
after the other without removing the calibration slide.
1. Mount the EVOS ™
(you can use either of the two slots).
2. Select the objective to calibrate using the Objective selection wheel (page 6).
3. Go to the Settings  Objectives tab, then click the Calibrate icon next to
the selected objective to launch the Objective Calibration tool.
™ M5000 Imaging System (Cat. No. AMEP4720). Total
Calibration Slide face down in the vessel holder for slides
™ M5000 Imaging
M5000 calibration slide
™
EVOS M5000 User Guide
™