Thermo Scientific EVOS M5000 User Manual page 41

EVOS M5000 User Guide
™
12. Enter the Total Time for Run 1 into the corresponding Hours, Minutes, and
Seconds fields. You can create multiple Runs for a Time Lapse Routine, each
with its own duration and image capture frequency.
13. Set the Image capture options for Run 1. Available options are:
•
Frequency: Enter the time period in Hours, Minutes, and Seconds that
must elapse before a new set of images are captured.
For example, in an experiment with an image capture frequency of
2 minutes and 30 seconds, the images will be captured every 2 minutes
and 30 seconds after the initial set of images are acquired at time point 0.
•
Intervals: Enter the total number of time intervals between the captured
image sets for a given run duration.
Images are collected at the end of an interval. For example, in a Run
experiment with a duration of 5 minutes and 2 intervals, the images will
be captured every 2 minutes and 30 seconds after the initial set of images
are acquired at time point 0.
•
As fast as possible: Select this option to capture a new set of images
immediately after completing the previous set without any delay between
the sets.
The speed with which the images are captured depends on the specific
choices made for the routine such as the autofocus frequency and
exposure settings.
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