Thermo Scientific EVOS M5000 User Manual page 32

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10. The Refine section displays a histogram plot showing Count versus Intensity,
Area, or Circularity. In this example, Intensity is selected.
11. To refine your count, select Intensity, Area, or Circularity, then move the
handles of the gate bars in the desired direction to set the upper or the lower
boundary for the selected parameter.
The software applies the selected boundaries and recalculates the count.
12. When finished with the count, save your count results (see "Save count
results", page 33).
IMPORTANT! If you navigate away from the Auto Count screen, your count will
be lost. To preserve the count results, make sure to save it before you navigate
away from the count screen.
EVOS M5000 User Guide
™