Thermo Scientific EVOS M5000 User Manual page 72

Z-Stack: Opens the Z-Stack tool, which allows you to capture
multiple images along the Z-axis based on your specifications. You
can use the images captured with the Z-Stack tool to generate a
Z-Stack Projection.
Save: Opens the Save dialog, which allows you to select a save
location, set save options, and enable the Quick Save option.
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