Thermo Scientific EVOS M5000 User Manual page 20

Focus on the
sample
Optional
: Configure
display settings
18
1. Use the Coarse and Fine focus sliders to focus on the sample.
Alternatively, click Coarse and Fine autofocus to autofocus on the sample.
2. To limit the autofocus algorithm to a specific region along the Z-axis, use the
Autofocus Maximum and Autofocus Minimum controls (blue triangles on
the Coarse focus slider) to set the upper and lower bounds.
Note: You can also focus on the sample manually using the focusing knobs
on the instrument (page 6).
Click Enable focus knob, then turn the focusing
knob until the region of interest is in focus.
3. Repeat the focus procedure for each channel you want to capture.
4. If you want to preserve the Z-Offsets between the channels, select
Lock Z.
•
When locked, the movement of the Z-axis in one channel affects all
channels.
•
When unlocked, only the current channel is affected.
Note: Z-Offsets specify the optimal focus position in a channel relative to the
focus position in other channels. Setting the correct Z-Offsets is especially
important when the fluorescent markers in different channels are in different
focal planes.
1. Hover the pointer over the Viewing area to reveal the buttons for Display
Settings and Analysis Tools.
2. Click Image Display Settings button to expand the controls for image
display parameters for the selected channel.
3. Adjust Brightness
sliders.
4. Click the Reset button
5. Click the Image Display Settings button again to collapse the controls.
, Contrast , and Gamma
to return the display settings to their default values.
with the corresponding
EVOS M5000 User Guide
™