Thermo Scientific EVOS M5000 User Manual page 39

For fluorescence and transmitted light applications

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EVOS

M5000 User Guide

™

Adjust brightness and focus for each selected channel as described in "Capture

images" (page 16), then click Next.

Note: When the sample is focused and ready, tighten the stage brakes (page 6)

to prevent the stage from drifting during the time lapse routine.

If you have opted to run Auto Focus, select the Auto Focus Channel to use.

Note: In a time lapse routine, you can run the autofocus procedure only in a

single channel (in this example, DAPI). The focal plane identified in this

channel is then used for all other channels.

Select to autofocus at Every interval or at

First interval of each run, then click Next.

Note: In a time lapse routine, images are captured at the end of each interval

of a run. For more information, see "Intervals", page 39.

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