Thermo Scientific EVOS M5000 User Manual page 30

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3. To identify the target objects to include in your count,
click Target, then click and drag to draw a circle (green)
around a representative target.
Note: For best results, follow these guidelines when identifying target objects:
•
When selecting objects, circle the entire object and include a slight border
around it.
•
To include objects of lower intensity in your count, select dimmer objects
during identification.
•
Circle only one object at a time to help define object size for segmentation.
4. If needed, click Target again to identify further objects to include in your count
(for example, targets that might appear different).
5. To distinguish the target from the background, click
Backround, then click and drag to draw a circle (red) in
a background area.
6. If needed, click Background again to identify other background areas or
background objects that might affect your count results.
EVOS M5000 User Guide
™