Thermo Scientific EVOS M5000 User Manual page 69

Brightness mode: Toggles between Simple and Actual modes for brightness controls.
• Simple mode allows you to control light intensity as a single Light parameter.
• Actual mode allows you to adjust Brightness (i.e., LED intensity), Exposure, and Gain parameters
individually.
Brightness controls: Adjust the brightness for the selected channel.
Current Z (µm): Displays the current focus position in µm along the Z-axis.
To change the Z-axis focus position, enter the desired value into the text box.
Autofocus (Coarse and Fine): Runs the coarse and fine autofocus
algorithms in the selected channel.
Coarse and Fine focus sliders: Allow you to adjust the focus in the selected channel
when in the Live mode.
Using the Autofocus Maximum and Autofocus Minimum controls (blue triangles on
the Coarse focus slider), you can limit the autofocus algorithm to the selected region
along the Z-axis.
Saved Settings: Allows you to save and edit Z-axis focus positions and
brightness settings for future use.
Press the button to save the current settings.
Lock Z: Allows you to lock the Z-axis offsets, which specify the optimal focus position in each
channel relative to the focus position in other channels.
• When locked, movement of the Z-position in one channel changes the Z-position in all channels,
preserving their relative Z-positions.
• When unlocked, adjusting the Z-position only affects the currently selected channel.
Enable focus knob: Enables the manual focus knobs.
Capture: Captures an image using the current capture settings and stores it in the image
cache of the channel in which it was captured.
You can select multiple channels to capture by selecting the
corresponding channel checkboxes.
Thumbnails: Display the most recently
captured image stored in the memory buffer
for the corresponding channel.
EVOS M5000 User Guide
™
Simple mode Actual mode
67