Thermo Scientific EVOS M5000 User Manual page 31

For fluorescence and transmitted light applications
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EVOS
M5000 User Guide
7.
To count closely grouped objects that are touching or overlapping as distinct
objects, select from the Split Cells options:
-
None: Touching or overlapping objects are not counted separately.
-
Shape: Distinct objects are identified based on shape.
-
Intensity: Distinct objects are identified based on intensity.
8.
When you are finished defining the target and background areas, the software
automatically counts the objects based on your examples.
Object Count field displays the number of objects included in the count.
Viewing area identifies the objects that were counted with a colored circles.
9.
To change the color of the circles that identify the objects
included in the count, select the desired color from the
Count Color dropdown.
29

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