Related Manuals for Thermo Scientific iBright CL750
Summary of Contents for Thermo Scientific iBright CL750
- Page 1 ™ iBright CL750 Imaging System USER GUIDE ™ For use with the iBright CL750 Imaging System Catalog Numbers A44116 Publication Number MAN0018652 Revision C.0 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256 For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC.
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Page 3: Table Of Contents
Contents ■ Product information ..............6 Product description . - Page 4 Contents ■ Export and analyze images ............21 Export images .
- Page 5 Contents Generate an audit history ............42 View the e-Signature history .
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Page 6: Product Information
Product information IMPORTANT! Before using this product, read and understand the information in the “Safety” appendix in this document. Product description ™ The iBright CL750 Imaging System allows users to image chemiluminescent Western blots, DNA and RNA gels stained with fluorescent nucleic acid stains, and visible protein gels. - Page 7 Product information Contents Table 2 Available accessories Component Part number White transilluminator screen A33828 Sample stage A33829 High-Power USB Wi-Fi Module A26774 iBright ™ CL750 Imaging System User Guide...
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Page 8: Start, Sign On, And Configure The Instrument
Start, sign on, and configure the instrument Create an instrument profile 1. Touch to sign in to your instrument user account. 2. Touch Get started. 3. Touch Create a Profile. 4. Enter a Screen name. 5. Enter and confirm a four-digit pin. 6. -
Page 9: Mobile Device
Start, sign on, and configure the instrument Create an instrument account and link to Connect using a cloud account Mobile device 1. Touch Mobile Device. 2. Launch instrument Connect mobile app. 3. Sign into your Connect account on the mobile app. 4. -
Page 10: Use Instrument To Link To Connect Account
Start, sign on, and configure the instrument Link to Connect using an existing instrument account Use instrument to link to Connect account 1. Click on Instrument. 2. Enter your Connect username and password. 3. Click Link account. Your Connect account should now be linked to your instrument account. Link to Connect using an existing instrument account Linking to Connect after account creation can only be done during the export process. -
Page 11: If The Unit Is On And In Sleep Mode
Start, sign on, and configure the instrument If the unit is on and in sleep mode 5. Open drawer and place sample in the center of the stage. 6. Close drawer. Touch Live View to see a live-view sample image. If the unit is on and in sleep mode 1. -
Page 12: Operate The Ibright ™ Imager
™ Operate the iBright imager Choose imaging mode and start image capture On the Welcome screen: 1. Use the drop-down menu to select the desired mode. • Chemi Blots to image chemiluminescent substrates. • Nucleic Acid Gels to image DNA and RNA gels. •... -
Page 13: Chemi Blots
Operate the iBright ™ imager Chemi Blots Chemi Blots Mode to image chemiluminescent Western blots. Image using Chemi Blots 1. Touch Smart Exposure. The imager acquires a series of short exposures, then renders a preview image and recommends an exposure time. This is not a real image. 2. -
Page 14: Nucleic Acid Gels
Operate the iBright ™ imager Nucleic Acid Gels Nucleic Acid Gels Mode to image DNA and RNA gels stained with fluorescent nucleic acid stains such ™ ™ ™ as Ethidium Bromide, SYBR Safe, SYBR Green (I or II), SYBR Gold and similar products. -
Page 15: Protein Gels
Operate the iBright ™ imager Protein Gels Protein Gels • Protein Gels Visible: USE WHITE SCREEN BETWEEN SAMPLE AND TRANSILLUMINATOR GLASS for samples including Coomassie-stained protein gels and silver-stained protein gels. • Protein Gels Fluorescent: PLACE SAMPLE DIRECTLY ON ™ TRANSILLUMINATOR GLASS for samples including No-Stain -lableled protein ™... -
Page 16: Universal Mode
Operate the iBright ™ imager Universal Mode Universal Mode Mode to image samples containing one or more signal types (chemi, fluor, and/or visible). Table 3 Illumination source, excitation filter, and emission filter for the ™ iBright CL750 Instrument. Illumination Excitation Filters Emission Filters Green trans 490-520 nm... -
Page 17: Options Available In Modes
Operate the iBright ™ imager Options available in modes 2. Touch Smart Exposure. Alternatively, for manual exposure, touch More options4Manual Exposure. The imager will acquire a series of short exposures for each dye type selected. It will then render preview images and a recommended exposure time for each channel. - Page 18 Operate the iBright ™ imager Options available in modes Table 5 Routines Suboption Detail Series of 5 preset exposure times producing one image for Multi-exposure each time period. Series of exposures where user defines the first capture (time interval), the last capture (cumulative capture time) and the total number of captures.
- Page 19 Operate the iBright ™ imager Options available in modes Table 7 Other options (continued) Suboption Detail Predicts optimal exposure for minimizing pixel saturation and maximizing dynamic range for a specific sample. Smart exposure Renders a preview of how the image will appear at the recommended exposure time.
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Page 20: Image Gallery
Operate the iBright ™ imager Image Gallery Image Gallery The Image Gallery is an on-board storage location where your image files are kept for future viewing. In the gallery you can easily retrieve, analyze and manage your image files. Any image acquired on the imager is automatically saved into a user's individual image gallery. -
Page 21: Export And Analyze Images
Export and analyze images Export images Export new images From the "Welcome" screen: 1. Capture an image. See “Choose imaging mode and start image capture” on page 12 2. Touch Export. 3. (Optional) Touch Edit to enter a new file name or comments. Touch Save. 4. -
Page 22: Select An Export File Type
Export and analyze images Export images 5. Choose a Destination. • Connect • • Network Drive • Print Note: The instrument requires a path for connection to a network drive or printer. Consult your IT department for questions regarding your network. The following information may be required to map a network drive: ·... -
Page 23: Archive/Backup G2I Image Files
Export and analyze images Export images Archive/Backup g2i image files ™ All iBright system users have an individual gallery for viewing and managing their ™ own image files. iBright administrators can manage all user galleries simultaneously or individually. 1. Touch Gallery. -
Page 24: Analyze Images
Export and analyze images Analyze images Analyze images Analyze new images 1. Capture an image. See “Choose imaging mode and start image capture” on page 12. 2. Touch Analyze. The system automatically finds and displays analysis frames, lanes and bands for the selected image file. - Page 25 Export and analyze images Analyze images After each image adjustment: a. Touch Apply to accept the changes. b. (Optional) Touch Next to display the analysis table. At the top of the table, touch to change the table output by: Output Units Description (Lane and Band Table) Volume...
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Page 26: Analyze Gallery Images
Export and analyze images Analyze images Analyze gallery images On the "Welcome" screen or capture screen Mode drop-down menu: 1. Touch Gallery or . Select one or multiple images. 2. Touch Actions. 3. If more than one image was initially selected, then choose one of the images within the scrollbar. -
Page 27: Maintain The Instrument
Maintain the instrument Settings Access Settings from the "Home" screen. Instrument Settings Provides all instrument details including serial number and About Instrument firmware version. Select a Time Zone, Date Format, and Time Format for Date and Time your region. Enable or disable a sleep mode by toggling the button. Sleep Mode Off = Guest users allowed. - Page 28 Maintain the instrument Settings File Naming Convention User sets preference for file naming by selecting up to 3 of the following aspects: Mode, Binning, Custom Field User, Exposure Time, and Sequential Number. Date and Time are mandatory aspects. The aspects can be ordered by choice. Error Log Displays instrument error history with sortable columns for Date, User, and Error Description.
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Page 29: Manage Accounts
Maintain the instrument Settings Service Tools Instrument Diagnostic Runs diagnostic testing on the instrument hardware. Provides an end report showing the functional status of hardware items. Note: Running diagnostics is not required. Contact a service technician for any issues. Ship Prep Mode Allows removal of the turntable for shipping. -
Page 30: Run Instrument Diagnostics
Maintain the instrument Run instrument diagnostics Run instrument diagnostics Instrument functions can be checked by a user to confirm proper hardware operating conditions. 1. Touch 2. Touch Service Tools. 3. Touch Instrument diagnostic. 4. Touch Start test. The instrument will check the instrument hardware for correct operation and settings. -
Page 31: Replace The Instrument Fuses
Maintain the instrument Replace the instrument fuses Replace the instrument fuses Materials required • Fuses, 1.6A, Time-Lag T, 250VAC, 5 × 20-mm (2) • Safety glasses • Powder-free gloves • Screwdriver, flathead Replace the fuses CAUTION! FIRE HAZARD. For continued protection against the risk of fire, replace fuses only with listed and certified fuses of the same type and rating as those ™... -
Page 32: Move The Instrument
Maintain the instrument Move the instrument 5. Install the fuse holder. 6. Plug in, then power on the iBright ™ imager. The installation is successful if the instrument powers on. ™ Note: Fuse failure can result from fluctuations in the supplied power to the iBright imager. -
Page 33: Prepare For Shipping
Maintain the instrument Move the instrument Prepare for shipping 1. Touch 2. Touch Service Tools. 3. Touch Ship prep mode. Drawer opens. 4. Remove turntable. 5. Touch Next. 6. Power off the instrument. iBright ™ CL750 Imaging System User Guide... -
Page 34: Invitrogen ™ Ibright ™ Sae Software Solution For 21 Cfr Part 11 Support
™ ™ Invitrogen iBright SAE Software Solution for 21 CFR Part 11 Support ™ Security Audit e-Signature (SAE) for the iBright Imager IMPORTANT! 21 CFR part 11 is a regulation that describes the criteria for acceptance by the U.S. Food and Drug Administration (FDA) for electronic records and electronic signatures. -
Page 35: Activate Your License Key
Invitrogen ™ iBright ™ SAE Software Solution for 21 CFR Part 11 Support Activate your license key Activate your license key 1. Log in as Local Admin on the instrument. IMPORTANT! Only the Local Administrator (Local Admin) has the ability to activate the license key on the instrument. -
Page 36: Disable Sae Mode
Invitrogen ™ iBright ™ SAE Software Solution for 21 CFR Part 11 Support Disable SAE mode 6. If not already done during the license activation, enter the IP address and port of the SAE console location and then touch Next. Note: IP address and port location (e.g., 10.X.X.X:8201/admin- console/index.html) are available in the address bar of the SAE Admin Console. -
Page 37: Change Your Password In Sae Mode
Invitrogen ™ iBright ™ SAE Software Solution for 21 CFR Part 11 Support Change your password in SAE mode Change your password in SAE mode 1. Touch User Profile on the left side of the top toolbar. 2. Touch Edit and enter your old password, your new password, and then confirm your new password. -
Page 38: Sae Privileges For Default User Roles
Invitrogen ™ iBright ™ SAE Software Solution for 21 CFR Part 11 Support SAE privileges for default user roles SAE privileges for default user roles Default Roles Category Function ™ ™ SAE Console iBright iBright Admin Admin Scientist Acquire, Analyze, Adjust, Export, and Image Management Delete Sign g2i File, Unlock g2i File... -
Page 39: Acquire Images In Sae Mode
Invitrogen ™ iBright ™ SAE Software Solution for 21 CFR Part 11 Support Acquire images in SAE mode Acquire images in SAE mode Image acquisition in SAE mode is the same as in non-SAE mode. ™ For iBright CL750 instruments, see the "Choose imaging mode and start image ™... -
Page 40: Commit Changes
Invitrogen ™ iBright ™ SAE Software Solution for 21 CFR Part 11 Support Commit changes 3. (Optional) Check the box Include guest's images to include the guest gallery. 4. Check the Images box for all users to view images from all user galleries. 5. -
Page 41: Sign Data
Invitrogen ™ iBright ™ SAE Software Solution for 21 CFR Part 11 Support Sign data 4. Touch Save. If successful, a window will show Changes committed. Note: Once complete, the file status changes from Uncommitted (X) to Committed ( ) and a new audit version is added to the audit history. The Commit button is dynamic and once changes are committed, it changes to Sign Data (see “Sign data”... -
Page 42: Unlock Files
Invitrogen ™ iBright ™ SAE Software Solution for 21 CFR Part 11 Support Unlock files Unlock files In SAE mode, signed files are locked and must be unlocked by a user with unlock privileges before any further changes can be made to the file. When a file is unlocked, the file’s e-Signature status changes to inactive. -
Page 43: View The E-Signature History
Invitrogen ™ iBright ™ SAE Software Solution for 21 CFR Part 11 Support View the e-Signature history View the e-Signature history Touch e-Signature history to review the e-Signature history. Note: The e-Signature History contains the following information: Commit version, Signed Date (and time), Signed By, Signed Role, Meaning, and Status. Note: e-Signature History is also available in the Adjust and Analyze workflows under More options. -
Page 44: Instrument Specifications
Instrument specifications Instrument dimensions and specifications Specification ™ iBright Imager Dimensions (L × W × H) 68.0 × 38.2 × 59.8 cm Weight 52 kg (115 lbs) Instrument clearance (Back) 15.25 cm (6 in.) Electrical requirements WARNING! For safety, the power outlet used for powering the instrument must be accessible at all times. -
Page 45: Environmental Requirements
Instrument specifications Environmental requirements Environmental requirements Condition Acceptable range Installation site Indoor use only Electromagnetic Do not use this device in close proximity to sources of strong interference electromagnetic radiation (for example, unshielded intentional RF sources). Strong electromagnetic radiation may interfere with the proper operation of the device. -
Page 46: Network Requirements
Instrument specifications Network requirements Network requirements The instrument: • Is factory-configured for IPv4 TCP/IP communication and includes an Ethernet adapter (100/1,000 Mbps) with an RJ45-type connector for integrating the device into a local area network (LAN). • Can alternatively be configured for wireless networking (High Power USB WiFi Module required, sold separately as an optional accessory). -
Page 47: Instrument And Computer Connections
Instrument specifications Instrument and computer connections Instrument and computer connections ™ iBright Imager front view Touchscreen USB port Sample stage iBright ™ CL750 Imaging System User Guide... - Page 48 Instrument specifications Instrument and computer connections iBright ™ Imager rear view USB port Ethernet port — RJ45 port for 10/100 Mbps ethernet communication with the instrument Fuse cover Power switch Power port — 100-240 VAC iBright ™ CL750 Imaging System User Guide...
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Page 49: Safety
Safety WARNING! GENERAL SAFETY. Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document. -
Page 50: Safety Alerts On This Instrument
Safety Symbols on this instrument (continued) Symbol English Français Do not dispose of this product in Ne pas éliminer ce produit avec les unsorted municipal waste déchets usuels non soumis au tri sélectif. CAUTION! To minimize MISE EN GARDE ! Pour negative environmental impact from disposal of electronic minimiser les conséquences... -
Page 51: Location Of Safety Labels On The Instrument
Safety Safety information for instruments not manufactured by Thermo Fisher Scientific Location of safety labels on the instrument Safety information for instruments not manufactured by Thermo Fisher Scientific Some of the accessories provided as part of the instrument system are not designed or built by Thermo Fisher Scientific. -
Page 52: Physical Injury
Safety Instrument safety Physical injury CAUTION! Moving Parts. Moving parts can crush, pinch and cut. Keep hands clear of moving parts while operating the instrument. Disconnect power before servicing. LED (Light-Emitting Diode) CAUTION! LED (light-emitting diode) HAZARD. Removing the protective covers and (when applicable) defeating the interlock(s) may result in exposure to the internal LED. -
Page 53: Electrical Safety
Safety Instrument safety Electrical safety WARNING! Ensure appropriate electrical supply. For safe operation of the instrument: · Plug the system into a properly grounded receptacle with adequate current capacity. · Ensure the electrical supply is of suitable voltage. · Never operate the instrument with the ground disconnected. Grounding continuity is required for safe operation of the instrument. -
Page 54: Cleaning And Decontamination
Safety Instrument safety Cleaning and decontamination CAUTION! Cleaning and Decontamination. Use only the cleaning and decontamination methods specified in the manufacturer's user documentation. It is the responsibility of the operator (or other responsible person) to ensure the following requirements are met: ·... -
Page 55: Safety And Electromagnetic Compatibility (Emc) Standards
Safety Safety and electromagnetic compatibility (EMC) standards Safety and electromagnetic compatibility (EMC) standards The instrument design and manufacture complies with the following standards and requirements for safety and electromagnetic compatibility. Safety compliance Reference Description EU Directive European Union “Low Voltage Directive” 2014/35/EU IEC 61010-1 Safety requirements for electrical equipment for measurement,... -
Page 56: Environmental Design
Safety Safety and electromagnetic compatibility (EMC) standards (continued) Reference Description ICES-001, Issue 4 Industrial, Scientific and Medical (ISM) Radio Frequency Generators FCC Part 15 Subpart B (47 CFR) U.S. Standard Radio Frequency Devices This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules. - Page 57 Safety Safety and electromagnetic compatibility (EMC) standards (continued) 产品中有害物质的名称及含量 China EEP Hazardous Substances Information 电源供应器 Power Supply 电机组件 Electromech anical Assemblies 本表格依据 SJ/T11364 的规定编制 This table is compiled according to SJ/T 11364 standard. O: 表示该有害物质在该部件所有均质材料中的含量均在 GB/T26572 规定的限量要求以下. Indicates that the concentration of the hazardous substance in all homogeneous materials for the part is below the relevant threshold of the GB/T 26572 standard.
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Page 58: Chemical Safety
Safety Chemical safety Chemical safety WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below. Consult the relevant SDS for specific precautions and instructions: · Read and understand the Safety Data Sheets (SDSs) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials. -
Page 59: Biological Hazard Safety
Safety Biological hazard safety · Limiter l’inhalation des produits chimiques. Ne pas laisser les récipients de produits chimiques ouverts. Ils ne doivent être utilisés qu’avec une ventilation adéquate (par exemple, sorbonne). · Vérifier régulièrement l’absence de fuite ou d’écoulement des produits chimiques. En cas de fuite ou d’écoulement d’un produit, respecter les directives de nettoyage du fabricant recommandées dans la FDS. -
Page 60: Documentation And Support
Documentation and support Related documentation and references Product Details Product Description Webpage URL iBright ™ Analysis Software Cloud-based iBright ™ Analysis Software http://thermofisher.com/ Connect Connect is supplied with one free terabyte of Connect digital data storage. ™ ™ iBright Imager Firmware Firmware updates and patches for all iBright Instruments are available for download on the Thermo Fisher website. -
Page 61: Customer And Technical Support
Documentation and support Customer and technical support Customer and technical support Visit thermofisher.com/support for the latest service and support information. • Worldwide contact telephone numbers • Product support information – Product FAQs – Software, patches, and updates – Training for many applications and instruments •... - Page 62 CL750 Instrument User Guide_UG_MAN0018652-v3-GUID-A95B1212-50E5-4547-94AC- D070C8471F1C-2020/07/21 18:10:35 en 17:12:14.381+01:00 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 24 August 2020...