Thermo Scientific EVOS M5000 User Manual page 65

For fluorescence and transmitted light applications
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EVOS
M5000 User Guide
4.
Find a calibration circle that fits entirely in the field of view.
5.
Focus on the calibration circle using the focus controls.
6.
Click and drag the green calibration lines to align them along the outer
borders of the calibration circle.
7.
When finished, click Save to complete the calibration. Repeat the calibration
process for each additional objective to be calibrated.
8.
When finished calibrating all of the installed objectives, click Done.
63

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