Thermo Scientific EVOS M5000 User Manual page 19

Adjust brightness
EVOS M5000 User Guide
™
1. For Brightness mode, select Simple or Actual.
-
Simple mode allows you to control Brightness as a single
parameter.
-
Actual mode allows you to adjust Light (i.e., LED intensity),
Exposure, and Gain parameters individually.
2. Adjust brightness using the Brightness controls:
•
In the Simple mode, move the Light slider to adjust brightness.
Alternatively, enter the desired brightness value (0%−100%) directly in the
brightness field.
•
In the Actual mode, move the Light (LED intensity), Exposure, and Gain
sliders to adjust the brightness parameters individually.
Alternatively, enter the desired value for each parameter (0%−100% for
Light, 0−4.00 seconds for Exposure, or 1.0−8.0 for Gain) directly in the
corresponding field.
Note: Optimize the brightness parameters as follows:
•
When searching for sample: Increase Gain for a brighter signal and
decrease Exposure for faster frame rate during navigation around the
vessel.
•
When capturing image: Decrease Gain to reduce background noise and
increase Exposure to regain signal intensity, as needed.
•
For brighter signal: Increase Light intensity for brighter illumination. If
needed, follow by increasing Gain.
•
For time lapse imaging: Increase Gain and Exposure, and decrease
Light intensity to reduce photobleaching and phototoxicity.
For example, for overnight time lapse experiments, capture one image
every 30 minutes or less, limit the use of autofocus, and use a channel
other than DAPI for autofocus.
•
In the Settings  Visuals tab, select Highlight Saturated Pixel to display
the overexposed pixels in the color of your choice. To obtain the
maximum level of brightness without any overexposed areas, dim the
illumination until the highlights disappear.
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