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RBC Bioscience MagCore HF48 User Manual page 41

Magcore automated nucleic acid extractor. magcore nucleic acid extraction kits

Operation manual - 24 pages

Troubleshooting

Symptoms

Low or NO DNA product

Poor PCR results

Clogging tip or liquid up to the tip filter

Comments and suggestions

1. The sample was lysed insufficiently. Make sure the

proteinase K was stored at -20 °C, and repeat the

procedure using fresh PK.

2. The sample was too large to lyse completely. The large

FFPE tissue was suggested cutting into 4 sections, and one

scroll was enough for extraction. Clogging tip will affect

the extraction process.

1. Poor quality FFPE samples. Fixation condition can affect

PCR performance, such as long-time storage in fixative.

2. DNA fragments. DNA purified from FFPE samples may

be fragmented due to formalin fixation, so we suggest

keeping amplicons as short as possible for PCR.

1. The sample was too large to pipetting. Large tissue

clogged the tip would result the liquid up to the tip filter or

the extraction cannot finish. We suggest cutting the tissue

before adding in the Heat Block well 1.

2. The sample was too much to pipetting. Do not extract too

much scrolls at a time. For large tissue, one scroll is enough

for extraction; for small tissue, we suggest not over 20mg

of FFPE.

MagCore®

Table of Contents

RBC Bioscience MagCore HF48 Operation Manual 24 pages