RBC Bioscience MagCore HF48 User Manual page 34
Magcore automated nucleic acid
extractor.
magcore nucleic acid extraction
kits
Hide thumbs
Also See for MagCore HF48
Operation manual
- 24 pages
Advertisement
Cigarette Butts Protocol
Sample preparation
2
1. Cut 1 cm
piece of outer paper from the end of the cigarette or filter. Cut this piece into 6 smaller pieces. Transfer
the pieces to a 1.5 ml microcentrifuge tube.
Cell Lysis
1. Add 500μl GT buffer and 20μl Proteinase K, close the lid, and mix for 10 sec. Incubate at 60°C for 1 hour to lyse the
sample.
2. Briefly centrifuge the tube to remove drops from the inside of the lid.
3. Pipette 400μl of clear tissue solution to the MagCore Sample Tube.
4. Place the prepared Sample Tube into well 4 of T-rack.
5. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
6. Run Code.401 program at MagCore®.
Hair roots Protocol
Sample preparation
1. Cut the hair roots into 0.5–1 cm pieces, and transfer them to the 1.5 ml microcentrifuge tube.
Cell Lysis
1. Add 500μl GT buffer and 20μl Proteinase K, close the lid, and mix for 10 sec. Incubate at 60°C for 1 hour to lyse the
sample.
2. Briefly centrifuge the tube to remove drops from the inside of the lid.
3. Pipette 400μl of clear tissue solution to the MagCore Sample Tube.
4. Place the prepared Sample Tube into well 4 of T-rack.
5. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
6. Run Code.401 program at MagCore®.
www.rbcbioscience.com
31
Advertisement
Also See for RBC Bioscience MagCore HF48
Related Manuals for RBC Bioscience MagCore HF48
-
Laboratory Equipment RBC Bioscience MagCore Plus II Service Manual
Automated nucleic acid extractor (80 pages)