RBC Bioscience MagCore HF48 User Manual page 34

Cigarette Butts Protocol
Sample preparation
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1. Cut 1 cm piece of outer paper from the end of the cigarette or filter. Cut this piece into 6 smaller pieces. Transfer
the pieces to a 1.5 ml microcentrifuge tube.
Cell Lysis
1. Add 500μl GT buffer and 20μl Proteinase K, close the lid, and mix for 10 sec. Incubate at 60°C for 1 hour to lyse the
sample.
2. Briefly centrifuge the tube to remove drops from the inside of the lid.
3. Pipette 400μl of clear tissue solution to the MagCore Sample Tube.
4. Place the prepared Sample Tube into well 4 of T-rack.
5. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
6. Run Code.401 program at MagCore®.
Hair roots Protocol
Sample preparation
1. Cut the hair roots into 0.5–1 cm pieces, and transfer them to the 1.5 ml microcentrifuge tube.
Cell Lysis
1. Add 500μl GT buffer and 20μl Proteinase K, close the lid, and mix for 10 sec. Incubate at 60°C for 1 hour to lyse the
sample.
2. Briefly centrifuge the tube to remove drops from the inside of the lid.
3. Pipette 400μl of clear tissue solution to the MagCore Sample Tube.
4. Place the prepared Sample Tube into well 4 of T-rack.
5. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
6. Run Code.401 program at MagCore®.
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