Cat.no.mgt-01 // Mgt - RBC Bioscience MagCore HF48 User Manual

Introduction of each MagCore® Nucleic Acid Extraction kit
MagCore® Genomic DNA Tissue Kit
For extraction of genomic DNA from a variety to animal tissues, paraffin-embedded tissue, swab, blood stain,
forensic specimens and cultured yeast.
Cartridge Code 401

Cat.No.MGT-01 // MGT-02

Kit Contents
Check that the following parts are included in addition to the main unit:
Cat.No. MGT-01 Contents:
Pre-filled Cartridge Reagent..............................................................................36 pcs.
Pipet Tip plus Holder Set.....................................................................................36 sets.
Sample Tube.............................................................................................................36 pcs.
Elution Tube...............................................................................................................36 pcs.
GT Buffer(30ml)..........................................................................................................1 pcs.
Filter Column Set.....................................................................................................36 pcs.
Proteinase K(11mg)..................................................................................................1 pcs.
PK Storage Buffer.......................................................................................................1 pcs.
Storage and Stability :
1. This kit should be stored at room temperature.
2. Proteinase K should be stored at -20°C when mixing with PK Storage Buffer.
3. Shelf life 12 months.
Cartridge Contents :
Description
MagCore® Genomic DNA Tissue Kit is designed for purification of total DNA (including genomic, mitochondrial and
viral DNA) from a variety of animal tissues or cells by using MagCore® auto-extraction instrument. The provided Filter
Column can filtrate hard tissue sample or swab sample to prevent tissue residues to obstruct pipette tip during the
process of MagCore® . The method uses pre-filled cartridge which contains proteinase K and a chaotropic salt to lyse
cells and degrade protein. DNA will bind to cellulose coated magnetic beads . After washing off the contaminants,
the purified DNA is eluted by low salt elution buffer. Purified DNA of approximately 20-30 kb in length is suitable for
PCR or other enzymatic reactions.
Applications
Using magnetic-particle technology to purify genomic DNA from animal tissues, paraffin embedded tissue, swab
and blood stain. The purified genomic DNA can be directly used for downstream application such as quantitative
PCR, restriction enzyme digestion, southern blotting... etc.
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Cat.No. MGT-02 Contents:
Pre-filled Cartridge Reagent..............................................................................96 pcs.
Pipet Tip plus Holder Set...................................................................................100 sets.
Sample Tube...........................................................................................................100 pcs.
Elution Tube............................................................................................................100 pcs.
GT Buffer(30ml)..........................................................................................................2 pcs.
Filter Column Set..................................................................................................100 pcs.
Proteinase K(11mg)..................................................................................................2 pcs.
PK Storage Buffer.......................................................................................................2 pcs.