RBC Bioscience MagCore HF48 User Manual page 31

Running Time:
33 min (sample volume :400 µl)
Preparation before using
1. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C.
For Paraffin-Embedded Tissue
Sample Preparation:
Additional Requirements: Xylene(or Substitutes), Ethanol (96-100%), Microcentrifuge Tube.
Suggested Xylene Substitute: A5597(Sigma), Neo-Clear(Merck), CitriSolv(Fisher).
1. Slice small section (5-10μm) of paraffin-embedded tissue and transfer to a microcentrifuge tube.
Discard the first 2-3 sections, if the surface of paraffin sample has been exposed to air.
2. Add 1ml xylene(or substitute) to the tube and vortex vigorously for 10sec. Then incubate at 60°C for 10min.
3. Centrifuge at full speed for 3min at room temperature.
4. Remove the supernatant carefully by pipetting, then add 1ml ethanol (96-100%) to the pellet and mix by vortexing for 10sec.
5. Centrifuge at full speed for 5min at room temperature.
6. Remove the supernatant carefully by pipetting, then add again of 1ml ethanol (96-100%) to the pellet and mix by vortexing
for 10sec to wash again.
7. Centrifuge at full speed for 5min.
8. Remove residual ethanol with a fine pipette tip, then open the tube and incubate at 55°C for 5min until all residual ethanol has
been evaporated.
9. Add 400μl GT Buffer and 20μl Proteinase K(10mg/ml) to the tube and mix by vortexing.
10. Incubate at 55°C for 90min until the sample has been completely lysed.
11. If there are any insoluble residues in the tube, transfer the supernatant to Filter Column and centrifuge at full speed for 5min to
get clear tissue solution in the Collection Tube.
12. Pipette 400μl of clear tissue solution to the MagCore Sample Tube.
13. Place the prepared Sample Tube into well 4 of T-rack.
14. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
15. Run Code.401 program at MagCore®.
For Swab Sample
Additional Requirements: PBS, Microcentrifuge Tube.
1. Separate the swab cotton form the stick. Place the swab into a 2ml microcentrifuge tube, add 500μl or more of GT Buffer and
20μl Proteinase K(10mg/ml).
2. Incubate the sample lyaste at 55°C for 30min.
For Buccal Swab sample, donor should not ingest anything for at least 30min prior to sample collection.
3. If there are any insoluble residues in the tube, transfer the supernatant to Filter Column and centrifuge at full speed for 5min to
get clear tissue solution in the Collection Tube.
4. Pipette 400μl of clear tissue solution to the MagCore Sample Tube.
5. Place the prepared Sample Tube into well 4 of T-rack.
6. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
7. Run Code.401 program at MagCore®.
MagCore®
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