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RBC Bioscience MagCore HF48 User Manual page 21

Magcore automated nucleic acid extractor. magcore nucleic acid extraction kits
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Running Time:
45 min (sample volume :200 µl) // 56 min (sample volume :400 µl)
Preparation before using
1. Add 1.0 ml RNase Free Water to the Carrier RNA tube and mix by vortexing. Store prepared Carrier RNA (1mg/ml) at -20°C.
2. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C.
Protocol
1. Pipet 10 μl Carrier RNA(1mg/ml) and 20 μl proteinase K(10mg/ml) into the MagCore Sample Tubes.
2. Add 200 μl or 400 μl of serum, plasma, or cell-free body fluids into the prepared Sample Tube.
3. Place the prepared Sample Tube into well 4 of T-rack.
4. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
5. Run Code.201 program at MagCore®.
Urine Protocol
Sample preparation
1. Harvest cells from up to 3.5 ml urine by centrifugation for 10 minutes at 3000 rpm and concentrate the sample to 400 ul
2. Add 5~10 ul ProteinaseK (10mg/ml) to the sample. Vortex for 5 seconds to mix sample.
3. Incubate at 56°C for 10 minutes until the sample lysate is clear. During incubation, invert the tube every 3 minutes.
4. Pipet 10 μl Carrier RNA(1mg/ml) into the MagCore Sample Tubes.
5. Place the prepared Sample Tube into well 4 of T-rack.
6. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
7. Run Code.201 program at MagCore®.
MagCore®
18

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