RBC Bioscience MagCore HF48 User Manual page 33

For Feed-soil Sample
1. Apply 30~40mg feed or soil samples into a 1.5 ml microcentrifuge tube.
2. Add 20μl (10mg/ml) Proteinase K and followed by adding 500μl of GT Buffer. Vortex gently until the powder suspend in GT
buffer.
3. Incubate the mixture at 56°C for 15mins. Invert the tube every 2~3mins during incubation.
Typically 15mins incubation can lysis more than 90% cells . Extend incubation time to 20mins can increase 10% of yield.
4. Centrifuge the mixture for 3 min at full speed.
5. If there are any insoluble residues in the tube, transfer the supernatant to Filter Column and centrifuge at full speed for 5min to
get clear tissue solution in the Collection Tube.
6. Pipette 400μl of clear tissue solution to the MagCore Sample Tube.
7. Place the prepared Sample Tube into well 4 of T-rack.
8. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
9. Run Code.401 program at MagCore®.
For Dried Blood Spot
1. Cut 3mm diameter punches from a dried blood spot with a single-hole paper punch. Place up to 3 blood card into a 1.5ml
microcentrifuge tube.
2. Add 400~500μl GT buffer into the microcentrifuge tube and continue to homogenize the sample tissue with grinding.
3. Add 20μl Proteinase K (10mg/ml) to the sample mixture and vortex to mix. Incubate at 60°C for 1 hour to lyse the sample.
4. If there are any insoluble residues in the tube, transfer the supernatant to Filter Column and centrifuge at full speed for 5min to
get clear tissue solution in the Collection Tube.
5. Pipette 400ul of clear tissue solution to the MagCore Sample Tube.
6. Place the Sample Tube into well 4 of T-rack.
7. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
8. Run Code.401 program at MagCore®.
Optional Step: RNA Degradation
If RNA-Free genomic DNA is required, perform these optional steps before adding Proteinase K.
1. Add 4μl RNase A(not provided, 50mg/ml) into the sample lysate.
2. Incubate the sample at room temperature for 20min.
MagCore®
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