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RBC Bioscience MagCore HF48 User Manual page 35

Magcore automated nucleic acid extractor. magcore nucleic acid extraction kits
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Chewing Gum Protocol
Sample preparation
1. Cut up to 30 mg of chewing gum into small pieces and transfer them to a 1.5 ml microcentrifuge tube.
Cell Lysis
1. Add 500μl GT buffer and 20μl Proteinase K, close the lid, and mix for 10 sec. Incubate at 60°C for 1~3 hours to lyse the sample.
2. Briefly centrifuge the tube to remove drops from the inside of the lid.
3. Pipette 400μl of clear tissue solution to the MagCore Sample Tube.
4. Place the prepared Sample Tube into well 4 of T-rack.
5. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
6. Run Code.401 program at MagCore®.
Betel Nut Residue Protocol
Sample preparation
1. Cut up to 30 mg of betel nut residue into small pieces and transfer them to a 1.5 ml microcentrifuge tube.
Cell Lysis
1. Add 500μl GT buffer and 20μl Proteinase K, close the lid, and mix for 10 sec. Incubate at 60°C for 1~3 hours to lyse the sample.
2. Briefly centrifuge the tube to remove drops from the inside of the lid.
3. Pipette 400μl of clear tissue solution to the MagCore Sample Tube.
4. Place the prepared Sample Tube into well 4 of T-rack.
5. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
6. Run Code.401 program at MagCore®.
MagCore®
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