━ Install Reagent Cartridge Please insert the front end of the cartridge into the space below the fixing plate of the Cartridge Rack. Please insert the Cartridge Rack before the T-Rack. ━ Install Tube, Tip 1. Put the tip into the corresponding well W5 Elution Tube W4 200μl SP Tip according to the left figure.
━ Installation Settings And Test Step 1. Please Set Up the Local Time. Step 2. Set the Instrument Nickname Go to Page 15 Step 3. Network Connection Settings Go to Page 15 Step 4. Test Piston Please refer to Piston Test chapter for information about the operation.
Touch Panel and User Interface MagCore® Plus II provides 7-inches full-color touch panel and easy-to-use user interface. Operator only needs to pay attention to the panel to know the current status, ending time and test report of the machine. The figure below shows the function list of the operation screen and the description of the status icons.
Operation Before Starting the Instrument Precautions When Opening the Front Door Plunged Upward from The Front Door Will For the sake of safety, the front the Lower Edge of the Rise Automatically door will not open to the top. Front Door Please push the front door up to raise it to the top when needed.
━ Emergency Stop Please read the security information to correctly stop the machine when an accident takes place. When an emergency situation occurs and the machine is operating, please press the stop button or open the front door to stop the machine immediately.
━ Start Programs Select DNase Treatment. (Only for RNA kit.) Please pretreat the sample according to the instructions of the user manual of the MagCore® Kit and put consumables into the machine. Select Sample Volume. Press Start to go to the next step. To Select User and Scan Barcodes: See Page 12 Select Elution Buffer.
━ Saved Settings ━ Access to Saved Settings After loading the Tips and Cartridges: If you need to save a customized setting, you can save it here and use the predetermined parameters for later uses. Press Saved Protocols on the start page After selecting the parameters, the confirmation of your settings will be displayed.
━ Set and Select Users Instrument can help you record the User Name as well as the Sample, Elution Tubes and Kit Lot No. used in the extraction for LIMS integration data. ━ Set User ━ Select User Name Select this icon if you want to add or edit a user Press the name block you want to select information.
━ Scan Sample / Elution Tube ━ Scan Kit Lot Select Scan Kit LOT Select Scan Sample or Scan Elution Tube The system will ask you if you want to delete all The system will ask you if you want to delete all the barcode data stored before.
━ Pause and Abort ━ Set Up the System Time You can pause or abort the current extraction while the program is in progress. Press the Pause button to pause at any time, and the machine will immediately suspend operation. This button is used as an emergency stop button.
━ Set the Instrument Nickname ━ Network Connection Settings You can give the instrument an easy-to-remember The network connection is used for the remote nickname for remote APP identification. monitoring APP and LIMS data transmission. To set the remote monitoring app, you need to first to check up with your IT staff that your network environment has DHCP service and can connect to the Internet and open the firewall 8083...
━ LIMS Data Collection There are two ways to get the data : 1. Get it via USB Flash Drive 2. Access Information through Internet Connection. 1. Get Data from USB Flash Drive Plug the USB Flash Drive into the instrument USB Open the Internet browser and enter the FTP Port.
Easy Maintenance Easy Maintenance includes the maintenance items which need to be done by users. These items include sterilization, clearing, simple test and error message report, etc. Users must perform these checkups periodically to ensure the system status is always good.
A. Test the Tip of the W2. B. Test the Tip of the W3 1. Please put the Tip Holder Set into the Well 2 of 1. Please put the Pipette Tip into Well 3 of the the T-Rack. T-Rack. 2.
C. Test the Tip of the W4 1. Please put the 200μl SP Tip into Well 4 of T-Rack. 2. Please add 1 ml water to the Sample Tube and put it in W1 of the T-Rack. 3. Please select the Test W4 Tip. 4.
━ Clean Piercing Needles ━ Eject Tip If the system is stopped from power shortage or other abnormal conditions, and the tips are still on the pistons, you have to press Reject Tip to remove the tips from the pistons. Please Select Reject Tip Button.
━ Heater Test ━ UV Sterilization Please Select Heater Test Button. Please Select the Sterilization Button. Please put the thermometer into the Heat Block Well 1 and press Next. Please select the sterilization time and press Start button. Warning! The UV lamp is dangerous. Please do not look straight at the inner of the machine when it is operating.
━ Alarm History ━ System Status If the machine malfunctions or the following You can check the system status and set up the messages appear, the engineers may ask you to system time in the menu. download the error records for them to diagnose the machine in advance.
━ Program Update ━ Clean the Waste Box You can use the USB to update the system. Please copy the downloaded program to the root directory of the USB Flash Drive. Then insert the Please dump the waste when the program is USB Flash Drive into the USB Port of the machine.
Advanced maintenance Advanced maintenance includes key-point maintenance, component replacement and advanced machine calibration. Only trained engineers can perform the above operations. Engineers recommended maintenance frequency Frequency of use Maintenance cycle Less than 20 times a month 1 Year 1 to 3 times daily Six months More than 3 times a day Three months...
━ Disassemble the housing 1. Loosen the two bottom screws to about 3mm.
2. Remove the remaining eight screws and take off the lid.
3. Move the cartridge/rack module inward, and loosen the two screws to about 3mm. Move the outlet metal sheet inward 3mm.
4. Remove the three screws on each side at the bottom of the case. 5.Move the sliding door upward to avoid the housing is stuck by the door sensor bar. 6. Remove the case by pulling out from the back of the machine.
━ Replace the pistons 1. Remove the screw on top of the fault piston. 2. Remove the eight screws on the metal sheet. 3. Remove the metal sheet. 4. Disassemble the fault piston then install a new one. 5. Screw back the top screw of the piston. 6.
━ Replace the ejector rod module 1. Remove the screws on top of the ejector rods on both sides. 2. Remove the springs. 3. Take out the ejector rod downward. Replace with a new ejector rod and screw back the top screw.
━ Replace the heater module Open the front door and take out the waste box, cartridge rack and T-rack. 1. Remove the middle screw. 2. Remove the temperature probe. 3. Remove the four screws on heater module. Install a new heater module and lock back all the parts.
━ Replace the X-axis motor belt 1. Remove the three screws of the metal sheet. 2. Remove the two screws of the metal plate from under the instrument. 3. Remove the metal plate, then replace with a new motor belt.
━ Replace the Y-axis motor 1. Unplug the connector of the Y-axis with the PCB panel. 2. Loosen the stop screw to about 1mm on the coupling. 3. Remove the four screws of the motor. Replace with a new Y-axis motor.
━ Replace M-axis motor 1. Remove the four screws on the magnetic module and take out the module. 2. Unplug the power connector of the lift motor. 3. Remove the four fixation screws of the motor. 4. Remove the two stop screws on the timing gear.
━ Replace the timing belt of the lift motor of the base 1. Remove the four screws on the magnetic module and take out the module. 2. Unplug the power connector of the lift motor. 3. Remove the two screws of the reinforced metal sheet of the motor.
━ Replace power supply 1. Loosen the four screws of power supply. 2. Remove the four screws inside the power supply. NOTE: Please contact RBC Bioscience for further questions regarding power supply replacement.
━ PLC Software Update 1.Tools for updating PLC software: (1.)a USB to RS232 adapter for DVP ES/EX/EH, or (2.)a RS232-PLC programming cable and a USB to RS232 converter. USB to RS232 adapter for RS232-PLC programming cable and a USB to RS232 converter DVP ES/EX/EH 2.Download PLC software update tool "EasyClient"from the link below and install.
4.Turn the switch from "Run" to "Stop" on PLC (marked in frame), plug the PLC-RS232 connection cable and connect with the computer, then turn on the machine power. 5. After the machine power is turned on, open “EasyClient”, then select “write DVZ into PLC”...
6.Click the folder (marked in circle), then select the PLC software for update, the filename extension is .dvz. 7.Select “Local (PLC COM Port)” and “COM Port” (please check COM Port number in device manager), select Baud Rate of 9600 bps, then click “Download”.
8.Download progress bar will be displayed if connected successfully, the downloading progress takes about 10 minutes. 9.After the download is completed, turn off the power, turn the switch from “Stop” to “Run” on PLC (marked in frame), unplug the PLC-RS232 connection cable (the cable linked with computer) and plug in the original interface cable.
10.Go to “System Status” and check the PLC version.
━ HMI Software Update 1.Copy HMI software to root directory of USB flash drive, insert the USB drive to the USB port. The HMI software update interface will open automatically after USB flash drive is detected, tap “Download”. 2.Enter password 111111 (six ones), check “Project”, then tap the folder icon.
3.Tap “usbdisk” then select the HMI software for update. The filename is “PLUS II-HMI (6 numbers) .cxob”, the file extension is .cxob, and the numbers indicate software version. 4.Tap “Download”, then reboot the machine. Unplug USB flash drive after go to homepage.
5.Go to “System Status” and check the HMI version.
━ Program Update 1.Download the latest version of program from RBC website, unzip the file on the computer. https://download.rbcbioscience.com/MagCore%C2%AE/ MagCore%C2%AE%20Automated%20Nucleic%20Acid%20 Extractor/MagCore%20Plus%20II/Software/ 2.There are 3 em files in the folder, please open the file em2, type the machine's S/N and save; you can find the S/N on the silver label on machine's housing, page “System Status”, and system teaching data sheet.
3.Copy the 3 files (em0, em1, and em2) to the root directory of a FAT32 formatted USB Flash Drive. 4.Tap “Maintenance”, tap next page, then insert USB. Please tap “Close” when you see a device detected pop-up. 5.Tap “Program Update”, then tap “Execute”.
6.Tap “Confirmation” when the program update is complete. 7.Go to “System Status” and check the program version.
━ Trouble Shooting for Alarm Code Content Causes and solutions DC Motor Up Time Out [Causes] The motor is not functional or the block plate of the sensor is at wrong position. [Solutions] (1) Check whether the motor is functional. (2) Check whether the cable dropped off.
Y_Encode no change [Causes] No signal response from the Encoder. [Solutions] (1) Replace the cable connected to the Encoder. (2) Replace the motor module. Y_Position Over Pulse [Causes] The position command coordinate greatly differ from the Encoder coordinate. [Solutions] (1) Check whether the terminal of the PLC Y00 Cable has dropped off. (2) Replace the cable connected to the power of the motor.
V_Encode no change [Causes] No signal response from the Encoder. [Solutions] (1) Replace the cable connected to the Encoder. (2) Replace the motor module. V_Position Over Pulse [Causes] The position command coordinate greatly differ from the Encoder coordinate. [Solutions] (1) Check whether the terminal of the PLC Y00 Cable has dropped off. (2) Replace the cable connected to the power of the motor.
X_Encode no change [Causes] No signal response from the Encoder. [Solutions] (1) Replace the cable connected to the Encoder. (2) Replace the motor module. X_Position Over Pulse [Causes] The position command coordinate greatly differ from the Encoder coordinate. [Solutions] (1) Check whether the terminal of the PLC Y00 Cable has dropped off.
Automated Nucleic Acid Extractor Model : Plus II Input Raitng :100-240V~ 50/60Hz, Max. 4.5AMax, 450W RBC Bioscience Corp. 3F, No.132. Lane 235, Baoqiao Rd., Xindian City, Taipei county 23145, T aiwan, R.O.C...