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RBC Bioscience MagCore HF48 User Manual page 25

Magcore automated nucleic acid extractor. magcore nucleic acid extraction kits
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Running Time: 90 min
Preparation before using
1. Add 1.0 ml RNase Free Water to the Carrier RNA tube and mix by vortexing. Store prepared Carrier RNA (1mg/ml) at -20°C.
2. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C.
Protocol
1. Pipet 20 μl Carrier RNA(1mg/ml) and 40 μl proteinase K(10mg/ml) into the 5ml Sample Tubes(provided).
2. Add 2400 μl of serum, plasma, or cell-free body fluids into the prepared 5ml Sample Tube.
3. Place the prepared 5ml Sample Tube into well 4 of T-rack(for 5ml Sample Tube).
4. Put the Elution Tube into well 1 of T-Rack and the Tip Plus Holder Set into well 2 of T-Rack.
5. Run Code.210 program at MagCore®.
(sample volume :2400 μl)
MagCore®
22

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