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RBC Bioscience MagCore HF48 User Manual page 39

Magcore automated nucleic acid extractor. magcore nucleic acid extraction kits
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Running Time:
175 min(2-hour heating) -Standard // 1012 min(16-hour heating)-High Yield
Preparation before using
1. Add 1.1 ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10 mg/ml) at -20°C.
For needle-like FFPE tissue slices
1. Add 500 μl Sula Oil, 20 μl PK and the FFPE tissue sample to the bottom of Heat Block well 1 of cartridge, and then cover it up
with the Thermostable cap.
Note: If the tissue is too large to lyse (the surface area over 300 mm
3") before adding in the heat block well 1 would be suggested. Make sure the tissue is at the bottom of the well to avoid
clipping it by Thermostable cap.
2. Place the Elution Tube into well 1 and the Tip Plus Holder Set into well 2 and 3 of T-Rack.
3. Run Code 405 program at MagCore®.
For glass-slide samples
1. Drop several Sula Oil on the glass slide and scrape them from the slide carefully, then put in the bottom of Heat Block well 1.
2. Add 500 μl Sula Oil and 20 μl PK into Heat Block well 1, rinse remaining sample on the wall and blade, then cover it up with
the Thermostable cap.
3. Place the Elution Tube into well 1, the Tip Plus Holder Set into well 2 and 3 of T-Rack.
4. Run Code 405 program at MagCore®.
Well
Elution Tube
Tip / Tip Holder
Tip / Tip Holder
2
) , cut it in 4 sections (Please see "important notes step
MagCore®
36

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