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RBC Bioscience MagCore HF48 User Manual

Magcore automated nucleic acid extractor. magcore nucleic acid extraction kits
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MagCore® HF48
MagCore® Super
MagCore® HF16Plus
Ver. 2016-2
www.rbcbioscience.com
MagCore® HF16 / HF48 / Compact
Nucleic Acid Extraction Kit
User's Manual
MagCore ®
Automated
Nucleic Acid
Extractor
MagCore® Nucleic Acid
Extraction Kits 96 Tests
RBC Bioscience Corp.
3F., No.132, Ln. 235, Baoqiao Rd., Xindian Dist.,
New Taipei City 23145, TAIWAN.
TEL : +886-2-8912-1200
EC REP
Wellkang Ltd (www.CE-marking.eu)
29 Harley St. London W1G 9QR, UK
MagCore®
Nucleic Acid
Extraction
Kits
MagCore® HF16
FAX : +886-2-8912-1300
MagCore® Compact
MagCore® Nucleic Acid
Extraction Kits 36 Tests

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  Summary of Contents for RBC Bioscience MagCore HF48

  • Page 1 MagCore® HF16Plus MagCore® Nucleic Acid Extraction Kits 96 Tests MagCore® Nucleic Acid Extraction Kits 36 Tests RBC Bioscience Corp. 3F., No.132, Ln. 235, Baoqiao Rd., Xindian Dist., New Taipei City 23145, TAIWAN. TEL : +886-2-8912-1200 FAX : +886-2-8912-1300 Ver. 2016-2 EC REP Wellkang Ltd (www.CE-marking.eu)
  • Page 3: Table Of Contents

    MagCore® HF16 / HF48 / Compact Nucleic Acid Extraction Kit User's Manual CONTENTS Precautions Product selection guide How to Use Kit Introduction of each MagCore® Nucleic Acid Extraction kits Kit Contents, Description, Applications, Pretreatment, Protocol MagCore® Genomic DNA Whole Blood Kit (Speedy installation) Cartridge Code 101 Cat.No.MGB400-01 // MGB400-02 MagCore®...
  • Page 4 Lysis Buffer or take appropriate measures according to local safety regulations. RBC Bioscience does not warrant that samples treated with Lysis Buffer are completely inactivated and noninfectious. After sample processing is completed, remove and autoclave all disposable plastics.
  • Page 5 MagCore® Product selection guide Genomic DNA Viral Nucleic Acids Total RNA...
  • Page 6: Cartridge Code

    How to use kit MagCore®HF16 and Compact ━ Install all necessary accessories and apply your specimen to MagCore®. ENSURE RACKS LOADED (1)CARTRIDGE RACK MAGCORE (2)T-RACK STAND-BY (ESC) PREV PAGE PRESS ( START ) TO RUN Press START. At this step prepare racks to operation area. After racks loaded then press Enter to next page for elution volume selection.
  • Page 7: Cartridge Code

    MagCore® How to use kit MagCore®HF48 ━ Start 48 Test 24 Test Start Cartridge Code Stop 105 Plasma DNA Extraction Kit (1.2 ml) Dnase Treatmen Sample Volume Save 200 µl Start Elution Buffer Elution Volume Load Program 150 µl Press START to Proceed Back When you finish input and press Start key, the system will show a confirmation window, on which there are the...
  • Page 8: Cartridge Code

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Genomic DNA Whole Blood Kit (Speedy installation) For purification of genomic DNA from human whole blood Cartridge Code 101 Cat.No.MGB400-01 // MGB400-02 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 9 MagCore® Running Time: 44 min (sample volume:200 μl) // 57 min (sample volume:400 μl) Whole Blood Protocol 1. Pipet 200/400μl of equilibrated whole blood sample to MagCore Sample Tube. 2. Put the prepared Sample Tube into well 4 of T-Rack. 3.
  • Page 10: Cartridge Code

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Genomic DNA Whole Blood Kit For purification of genomic DNA from human whole blood Cartridge Code 102 Cat.No.MGB400-03 // MGB-400-04 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 11 MagCore® Running Time: 44 min(sample volume :200 µl) // 57 min(sample volume :400 µl) Preparation before using 1. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C. Whole Blood Protocol 1.
  • Page 12: Cartridge Code

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Genomic DNA Large Volume Whole Blood Kit For purification of genomic DNA from human whole blood (1.2 ml) Cartridge Code 104 Cat.No.MGB1200 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 13 MagCore® Running Time: 76 min (sample volume :1200 µl) Preparation before using 1. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C. Protocol 1. Pipet Proteinase K 80 μl into the MagCore Sample Tubes. 2.
  • Page 14: Cartridge Code

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Plasma DNA Extraction Kit (1.2 ml) For extraction of free circulating DNA from human plasma or serum. Cartridge Code 105 Cat.No.MPD1200 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 15 MagCore® Running Time: 70 min (sample volume: 1200μl) Preparation before using 1. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C. Protocol 1. Pipet 20 μl proteinase K(10mg/ml) into the MagCore Sample Tubes. 2.
  • Page 16: Cartridge Code

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Genomic DNA Whole Blood Kit (For Genotyping) Purify genomic DNA from human whole blood for genotyping. Cartridge Code 106 Cat.No.MGB400-07 // MGB-400-08 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 17 MagCore® Running Time: 44 min(sample volume :200 µl) // 57 min(sample volume :400 µl) Preparation before using 1. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C. Whole Blood Protocol 1.
  • Page 18: Cartridge Code

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Cultured Cells DNA Kit For extraction of genomic DNA from cultured cells and amniotic fluid. Cartridge Code 110 Cat.No.MCC-01 // MCC-02 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 19 MagCore® Running Time: 44 min (sample volume: 200 µl, up to 5 x 10 cells) Preparation before using 1. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C. 2.
  • Page 20: Cartridge Code

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Viral Nucleic Acid Extraction Kit For extraction of viral DNA/RNA from serum, plasma and cell-free body fluids. Cartridge Code 201 Cat.No.MVN400-01 // MVN-400-02 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 21 MagCore® Running Time: 45 min (sample volume :200 µl) // 56 min (sample volume :400 µl) Preparation before using 1. Add 1.0 ml RNase Free Water to the Carrier RNA tube and mix by vortexing. Store prepared Carrier RNA (1mg/ml) at -20°C. 2.
  • Page 22: Cartridge Code

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Viral Nucleic Acid Extraction Kit For extraction of viral DNA/RNA from serum, plasma and cell-free body fluids Cartridge Code 202 Cat.No.MVN400-03 // MVN-400-04 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 23 MagCore® Running Time: 57 min (sample volume :200 µl) // 66 min(sample volume :400 µl) Preparation before using 1. Add 1.0 ml RNase Free Water to the Carrier RNA tube and mix by vortexing. Store prepared Carrier RNA (1mg/ml) at -20°C. 2.
  • Page 24: Cartridge Code

    MagCore® Viral Nucleic Acid Large Volume Extraction Kit MagCore® Viral Nucleic Acid Large Volume Extraction Kit (2.4 ml) For extraction of viral DNA/RNA from large volume (2.4 ml) serum, plasma and cell-free body fluids. Cartridge Code 210 Cat.No.MVN2400 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 25 MagCore® Running Time: 90 min (sample volume :2400 μl) Preparation before using 1. Add 1.0 ml RNase Free Water to the Carrier RNA tube and mix by vortexing. Store prepared Carrier RNA (1mg/ml) at -20°C. 2. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C. Protocol 1.
  • Page 26: Cartridge Code

    MagCore® Viral Nucleic Acid Large Volume Extraction Kit MagCore® Viral Nucleic Acid Large Volume Extraction Kit (1.2 ml) For extraction of viral DNA/RNA from large volume (1.2 ml) serum, plasma and cell-free body fluids. Cartridge Code 211 Cat.No.MVN1200 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 27 MagCore® Running Time: 73 min (sample volume :1200 μl) Preparation before using 1. Add 1.0 ml RNase Free Water to the Carrier RNA tube and mix by vortexing. Store prepared Carrier RNA (1mg/ml) at -20°C. 2. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C. Protocol 1.
  • Page 28: Cat.no.mgp-01 // Mgp

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Genomic DNA Plant Kit For extraction of genomic DNA from plant and fungal tissues. Cartridge Code 301 Cat.No.MGP-01 // MGP-02 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 29 MagCore® Running Time: 33min(sample volume: 400 µl) Preparation before using The kit procedures are optimized for a maximum of 100 mg of wet-weight or 20 mg of dried starting material. Exceeding the recommended maximum amount of starting material will result in inefficient lysis, resulting in low yield and purity.
  • Page 30: Cat.no.mgt-01 // Mgt

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Genomic DNA Tissue Kit For extraction of genomic DNA from a variety to animal tissues, paraffin-embedded tissue, swab, blood stain, forensic specimens and cultured yeast. Cartridge Code 401 Cat.No.MGT-01 // MGT-02 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 31 MagCore® Running Time: 33 min (sample volume :400 µl) Preparation before using 1. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C. For Paraffin-Embedded Tissue Sample Preparation: Additional Requirements: Xylene(or Substitutes), Ethanol (96-100%), Microcentrifuge Tube. Suggested Xylene Substitute: A5597(Sigma), Neo-Clear(Merck), CitriSolv(Fisher).
  • Page 32 For Solid Animal Tissue Additional Requirements: Microcentrifuge Tube. 1. Cut the solid tissue to small pieces (up to 30 mg) and put into a microcentrifuge tube. 2. Add 400μl GT Buffer and 20μl Proteinase K(10mg/ml) to the tube and mix by vortexing. 3.
  • Page 33 MagCore® For Feed-soil Sample 1. Apply 30~40mg feed or soil samples into a 1.5 ml microcentrifuge tube. 2. Add 20μl (10mg/ml) Proteinase K and followed by adding 500μl of GT Buffer. Vortex gently until the powder suspend in GT buffer. 3.
  • Page 34 Cigarette Butts Protocol Sample preparation 1. Cut 1 cm piece of outer paper from the end of the cigarette or filter. Cut this piece into 6 smaller pieces. Transfer the pieces to a 1.5 ml microcentrifuge tube. Cell Lysis 1. Add 500μl GT buffer and 20μl Proteinase K, close the lid, and mix for 10 sec. Incubate at 60°C for 1 hour to lyse the sample.
  • Page 35 MagCore® Chewing Gum Protocol Sample preparation 1. Cut up to 30 mg of chewing gum into small pieces and transfer them to a 1.5 ml microcentrifuge tube. Cell Lysis 1. Add 500μl GT buffer and 20μl Proteinase K, close the lid, and mix for 10 sec. Incubate at 60°C for 1~3 hours to lyse the sample. 2.
  • Page 36 Saliva Protocol Sample preparation 1. For saliva sample, donor should not ingest anything for at least 30min prior to sample collection. 2. Prepare PBS Buffer and 15 ml tube. Cell Lysis 1. Apply the 1 ml saliva and add 4 ml PBS buffer (not provided). 2.
  • Page 37 MagCore® Cultured Yeast Protocol • Additional requirements: Sorbitol Buffer, Lyticase or Zymolase, Microcentrifuge tube. • Preparation of Sorbitol Buffer: 1.2 M sorbitol, 10mM CaCl2, 0.1M Tris-Cl pH 7.5. Sterilize by filtration and store at 4°C Sample preparation 1. Harvest 3ml yeast cells (up to 5x10 cells) by centrifugation at 5000 x g for 10 minutes.
  • Page 38: Mgf-01 // Mgf

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Genomic DNA FFPE One-Step Kit For extraction of total DNA from formalin-fixed paraffin-embedded (FFPE) tissue by using MagCore® System. Cartridge Code 405 Cat. No. MGF-01 // MGF-03 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 39 MagCore® Running Time: 175 min(2-hour heating) -Standard // 1012 min(16-hour heating)-High Yield Preparation before using 1. Add 1.1 ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10 mg/ml) at -20°C. For needle-like FFPE tissue slices 1.
  • Page 40 Important Notes 1. The surface area of the FFPE tissue sample could be measured as following examples: Medium Large Small 0.8cm 1.5cm 1.7cm 1.2cm 1.2cm 2.1cm 2. Sample amount of preparation can be 1-5 scrolls, each with a thickness up to 5μm. One FFPE scroll could be enough to analyze if the surface area is over 200 mm Surface area (mm Sample scroll...
  • Page 41 MagCore® Troubleshooting Symptoms Comments and suggestions 1. The sample was lysed insufficiently. Make sure the proteinase K was stored at -20 °C, and repeat the procedure using fresh PK. Low or NO DNA product 2. The sample was too large to lyse completely. The large FFPE tissue was suggested cutting into 4 sections, and one scroll was enough for extraction.
  • Page 42: Cat.no.mbb-01 // Mbb

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Genomic DNA Bacterial Kit For extraction of genomic DNA from bacteria. Cartridge Code 502 Cat.No.MBB-01 // MBB-02 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 43 MagCore® Running Time: 44min (sample volume :200 µl) Preparation before using 1. Add 1.1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing. Store prepared Proteinase K (10mg/ml) at -20°C. 2. Freshly prepared 20mg/ml Lysozyme solution before use. (for Gream + bacteria isolation, Lysozyme solution is necessary) Lysozyme ( not provided ) + Lysozyme Reaction Buffer = Lysozyme Solution For Sputum Specimens Specimens Decontamination...
  • Page 44: Cat.no.mrn-01 // Mrn

    Introduction of each MagCore® Nucleic Acid Extraction kit MagCore® Total RNA Whole Blood Kit For total RNA extraction from human whole blood. Cartridge Code 601 Cat.No.MRN-01 // MRN-02 Kit Contents Check that the following parts are included in addition to the main unit: Cat.No.
  • Page 45 3. RNase-free DNase I is not including in MagCore total RNA Whole Blood Kit, we recommend to use RBC RNase-free DNase I (Cat#DN036 or Cat#DN096) for genomic DNA treatment. For product information, please contact RBC Bioscience distributor. We also recommend to use RNase-free DNase I enzyme(1U/μl) of Novagen (Cat#69182-3). Please contact local Merck branch office or distributor for product information.
  • Page 46: Cat.no.mrc-01 // Mrc

    MagCore® Total RNA Cultured Cells Kit MagCore® Total RNA Cultured Cells Kit For total RNA extraction from cultured cells. Cartridge Code 610 Cat.No.MRC-01 // MRC-02 For isolation of total RNA from animal tissues and FFPE samples, the MagCore total RNA Cultured Cells Kit is a relatively suitable choice and functions with a special protocol.
  • Page 47 3. RNase-free DNase I is not including in MagCore total RNA Cultured Cells Kit, we recommend to use RBC RNase-free DNase I (Cat#DN036 or Cat#DN096) for genomic DNA treatment. For product information, please contact RBC Bioscience distributor. We also recommend to use RNase-free DNase I enzyme(1U/μl) of Novagen (Cat#69182-3). Please contact local Merck branch office or distributor for product information.
  • Page 48 Running Time List Cat. Number Product Reactions Code No. Running Time MGB400-01 44 min (sample volume :200 μl) MagCore® Genomic DNA Whole Blood Kit (Speedy Installation) 57 min (sample volume :400 μl) MGB400-02 MGB400-03 44 min (sample volume :200 μl) MagCore®...
  • Page 49 MagCore® Ordering information Product Contents Cat. No. Accessories of HF16 Cartridge Rack MRC001 For general using MRC002 T-Rack For MVN2400 MRC003 T-Rack (for 5 ml sample tube) Enzyme 11 mg Proteinase K, Proteinase K Set PK011 1.25 ml PK Storage Buffer 50 μl RNase A (50mg/ml) RN050 RNase A...
  • Page 50 Note www.rbcbioscience.com...
  • Page 51 MagCore® Note...
  • Page 52 MagCore ® RBC Bioscience Corp. 3F, No.132, Lane 235, Baoqiao Rd, Xindian Dist, New Taipei City 23145, Taiwan TEL: +886-2-8912-1200 FAX: +886-2-8912-1300 http://www.rbcbioscience.com...