tecan NanoQuant Plate Quick Manual page 28

Calculations based on Individual Blanking
The well-specific absorbance value at 310 nm for Infinite M200 PRO and
Infinite M1000 (340 nm for Infinite F200 PRO and Infinite F500) is
subtracted from the corresponding absorbance value at 260 nm. Each well
used for sample measurement is then blanked individually with the
corresponding blanking value.
Infinite M200 PRO/Infinite M1000
Abs
A1
Abs
A2
Abs
B1
etc.
Infinite F200 PRO/Infinite F500
Abs
A1
Abs
A2
Abs
B1
etc.
The absorbance values at 280 nm are also corrected by the corresponding
absorbance values at 310 nm for Infinite M200 PRO and Infinite M1000
(or 340 nm for Infinite F200 PRO and Infinite F500). The corrected
absorbance values are used for the 260/280 ratio calculation.
28 Quick Guide NanoQuant Plate
= (Abs – Abs
260 A1 310 A1
= (Abs – Abs
260 A2 310 A2
= (Abs – Abs
260 B1 310 B1
= (Abs – Abs
260 A1 340 A1
= (Abs – Abs
260 A2 340 A2
= (Abs – Abs
260 B1 340 B1
) – Abs [OD]
blank average
) – Abs [OD]
blank average
) – Abs [OD]
blank average
) – Abs [OD]
blank average
) – Abs [OD]
blank average
) – Abs [OD]
blank average
TM
No.30035094 Rev No. 1.4 2013-06