tecan NanoQuant Plate Quick Manual page 26

Example
An absorbance value of 1 OD means a transmittance of 10%, and an
absorbance value of 2 OD means a transmittance of 1%, etc. Because
absorbance calculations are based on logarithmic dimensions, calculations
between absorbance sample values and absorbance blank values are
performed by subtraction and not by division. For more information review
the Lambert-Beer law.
4.2 Blanks
Average Blanking
The average absorbance value at 310 nm for Infinite M200 PRO and
Infinite M1000 (340 nm for Infinite F200 PRO and Infinite F500) of all wells
used for blanking is subtracted from the average absorbance value at
260 nm or 280 nm. The relative variation of the wells used for average
blanking must be below 10% in order to be able to start a measurement.
Infinite M200 PRO and Infinite M1000
Abs
blank average
Infinite F200 PRO and Infinite F500
Abs
blank average
Individual Blanking
The well-specific absorbance value at 310 nm for Infinite M200 PRO and
Infinite M1000 (340 nm for Infinite F200 PRO and Infinite F500) is
subtracted from the corresponding absorbance value at 260 nm.
Infinite M200 PRO/Infinite M1000
Abs
blank A1
Abs
blank A2
Abs
blank B1
etc.
26 Quick Guide NanoQuant Plate
= Abs
260 average
= Abs
260 average
= Abs – Abs
260 A1
= Abs – Abs
260 A2
= Abs – Abs
260 B1
– Abs [OD]
310 average
– Abs [OD]
340 average
[OD]
310 A1
[OD]
310 A2
[OD]
310 B1
TM
No.30035094 Rev No. 1.4 2013-06