tecan NanoQuant Plate Quick Manual page 27

Infinite F200 PRO/Infinite F500
Abs
blank A1
Abs
blank A2
Abs
blank B1
etc.
Individual blanking information is stored for each well used and blank-
correction of the samples is done with the corresponding single blanking
values instead of one average blank. Every well that is to be used for
sample measurement needs to be blanked beforehand.
4.3 Samples
Calculations based on Average Blanking
The well-specific absorbance value at 310 nm for Infinite M200 PRO and
Infinite M1000 (340 nm for Infinite F200 PRO and Infinite F500) is
subtracted from the corresponding absorbance value at 260 nm. Each well
used for sample measurement is then blanked with the average blanking
value.
Infinite M200 PRO/Infinite M1000
Abs = (Abs
A1
Abs = (Abs
A2
Abs = (Abs
B1
etc.
Infinite F200 PRO/Infinite F500
Abs = (Abs
A1
Abs = (Abs
A2
Abs = (Abs
B1
etc.
2013-06 Quick Guide NanoQuant Plate
= Abs – Abs
260 A1
= Abs – Abs
260 A2
= Abs – Abs
260 B1
– Abs ) – Abs
260 A1 310 A1
– Abs ) – Abs
260 A2 310 A2
– Abs ) – Abs
260 B1 310 B1
– Abs ) – Abs
260 A1 340 A1
– Abs ) – Abs
260 A2 340 A2
– Abs ) – Abs
260 B1 340 B1
[OD]
340 A1
[OD]
340 A2
[OD]
340 B1
[OD]
blank average
[OD]
blank average
[OD]
blank average
[OD]
blank average
[OD]
blank average
[OD]
blank average
TM
No.30035094 Rev No. 1.4 27